DAG1 Protein, Human (HEK293, His)

DAG1 Proteinas, a pivotal extracellular glycoprotein, is crucial for laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Serving as a receptor for laminin-G domain-containing extracellular matrix proteins, DAG1 interacts with laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells and acts as a receptor for laminin LAMA5. DAG1 Protein, Human (HEK293, His) is the recombinant human-derived DAG1 protein, expressed by HEK293 , with C-His labeled tag. The total length of DAG1 Protein, Human (HEK293, His) is 720 a.a., with molecular weight (glycosylation form) of ~95-110 kDa, 55-95 kDa, 17-20 kDa.

Dystroglycan (DAG1) plays a crucial role in various biological processes, participating in laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. As an extracellular peripheral glycoprotein, DAG1 serves as a receptor for extracellular matrix proteins containing laminin-G domains. Specifically, it acts as a receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells, and also functions as a receptor for laminin LAMA5.

Human

HEK293

C-His

Q14118 (H30-V749)

1605  [NCBI]

HWPSEPSEAVRDWENQLEASMHSVLSDLHEAVPTVVGIPDGTAVVGRSFRVTIPTDLIASSGDIIKVSAAGKEALPSWLHWDSQSHTLEGLPLDTDKGVHYISVSATRLGANGSHIPQTSSVFSIEVYPEDHSELQSVRTASPDPGEVVSSACAADEPVTVLTVILDADLTKMTPKQRIDLLHRMRSFSEVELHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLNQNSVPDIHGVEAPAREGAMSAQLGYPVVGWHIANKKPPLPKRVRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPGQIRPTMTIPGYVEPTAVATPPTTTTKKPRVSTPKPATPSTDSTTTTTRRPTKKPRTPRPVPRVTTKVSITRLETASPPTRIRTTTSGVPRGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDHEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDRAPARFKAKFVGDPALVLNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQIAGLSRRIAEDDGKPRPAFSNALEPDFKATSITVTGSGSCRHLQFIPVVPPRRVPSEAPPTEVPDRDPEKSSEDDV

Approximately 95-110 kDa, 55-95 kDa and 17-20 kDa due to glycosylation

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.