DARS Protein, Human (His)

DARS Protein, a central player, catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP, maintaining cellular energy homeostasis. It also exhibits nucleoside diphosphate kinase activity, producing various nucleoside triphosphates. At a low rate, DARS participates in thiamine triphosphate synthesis from thiamine diphosphate and ADP, showcasing its multifaceted role in nucleotide metabolism. DARS Protein, Human (His) is the recombinant human-derived DARS protein, expressed by E. coli , with N-His labeled tag. The total length of DARS Protein, Human (His) is 501 a.a., with molecular weight of ~47 kDa.

Adenylate Kinase 1 (AK1) protein plays a pivotal role in cellular energy homeostasis by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. Additionally, it exhibits nucleoside diphosphate kinase activity, enabling the production of various nucleoside triphosphates, including ATP, CTP, GTP, UTP, dATP, dCTP, dGTP, and dTTP, from their corresponding diphosphate substrates, using either ATP or GTP as a phosphate donor. Furthermore, AK1, though at a very low rate, participates in the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP, showcasing its multifaceted enzymatic activities in nucleotide metabolism.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-His

P14868 (M1-P501)

1615  [NCBI]

Approximately 47 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.