Delta-like protein 1/DLL1 Protein, Rat (HEK293, His)

Delta-like protein 1 (DLL1) is a transmembrane ligand for NOTCH1, NOTCH2, and NOTCH3 receptors and participates in cis- and trans-interactions with their extracellular domains. Upon interaction, DLL1 induces mechanical forces that promote Notch signaling by triggering NICD nuclear accumulation. Delta-like protein 1/DLL1 Protein, Rat (HEK293, His) is the recombinant rat-derived Delta-like protein 1/DLL1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Delta-like protein 1/DLL1 Protein, Rat (HEK293, His) is 517 a.a., with molecular weight of ~81.7 kDa.

Delta-like protein 1 (DLL1), a transmembrane ligand protein of NOTCH1, NOTCH2, and NOTCH3 receptors, engages in both cis and trans interactions with the extracellular domain (ECD) of Notch receptors. Following transinteraction, ligand cells induce mechanical force through clathrin-mediated endocytosis, ligand ubiquitination, EPN1 interaction, and actin polymerization. These events facilitate Notch receptor extracellular domain (NECD) transendocytosis, triggering Notch signaling by cleavage, hyperphosphorylation, and nuclear accumulation of the intracellular domain of Notch receptors (NICD). DLL1 is crucial for embryonic development and the maintenance of adult stem cells in various tissues and the immune system, regulating cell lineage, specification, patterning, and morphogenesis through differentiation and proliferation effects. It plays a vital role in brain development, modulating neuronal differentiation and neurogenesis in different contexts. DLL1 also contributes to immune system development, inhibiting B-cell lineage differentiation while promoting the emergence of T-cell/NK-cell precursors. Additionally, DLL1 regulates muscle development, pancreatic cell development, arterial identity maintenance, angiogenesis, goblet cell differentiation in the intestine, inner ear development, nephron development, and various physiological processes, including growth, blood pressure, and energy homeostasis. DLL1 interacts with several proteins, including TJP1, MAGI1, PSEN1, MFAP5, MIB1, NEURL1B, SYNJ2BP, MAGI2, MAGI3, MPDZ, and NOTCH1, playing a multifaceted role in diverse cellular processes.

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is approximately 1.431 μg/mL in the presence of 500 ng/mL of BMP-2, corresponding to a specific activity is 698.812 units/mg.

Rat

HEK293

C-10*His

P97677 (Q18-S534)

84010  [NCBI]

QVWSSGVFELKLQEFVNKKGLLGNRNCCRGGSGPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGAGIDPAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLTTQRHLTVGEEWSQDLHSSGRTDLRYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGERGEKMCDPGWKGQYCTDPICLPGCDDQHGYCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWGGLFCNQDLNYCTHHKPCRNGATCTNTGQGSYTCSCRPGYTGANCELEVDECAPSPCRNGGSCTDLEDSYSCTCPPGFYGKVCELSAMTCADGPCFNGGRCSDNPDGGYTCHCPAGFSGFNCEKKIDLCSSSPCSNGAKCVDLGNSYLCRCQTGFSGRYCEDNVDDCASSPCANGGTCRDSVNDFSCTCPPGYTGRNCSAPVSRCEHAPCHNGATCHQRGQRYMCECAQGYGGANCQFLLPEPPPDLIVAAQGGS

Approximately 55-60 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.