Delta-like protein 4/DLL4 Protein, Human (HEK293, Fc)

The DLL4 protein, a homolog of the Drosophila delta gene family, encodes Notch ligands with a DSL domain, EGF repeats, and a transmembrane domain. DLL4 is widely expressed in tissues, including placenta (RPKM 11.5), fat (RPKM 11.2), and 22 others. Delta-like protein 4/DLL4 Protein, Human (HEK293, Fc) is the recombinant human-derived Delta-like protein 4/DLL4 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Delta-like protein 4/DLL4 Protein, Human (HEK293, Fc) is 498 a.a., with molecular weight of 100-110 kDa.

Delta-like protein 4 (DLL4) is a homolog of the Drosophila delta gene family, encoding Notch ligands that possess a DSL domain, EGF repeats, and a transmembrane domain. DLL4 displays broad expression in various tissues, including placenta (RPKM 11.5), fat (RPKM 11.2), and 22 other tissues.

1. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells and the ED₅₀ is typically 2.2 µg/mL in the presence of 500 ng/mL recombinant human BMP2.
2. Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is approximately 0.05348 μg/ml in the presence of 100 ng/mL Mouse BMP-2, corresponding to a specific activity is 1.870×10^4 units/mg.

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  Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is approximately 0.05348μg/mL in the presence of 100 ng/mL Mouse BMP-2, corresponding to a specific activity is 1.870×10⁴ units/mg.

Human

HEK293

C-hFc

NP_061947.1 (S27-P524)

54567  [NCBI]

SGVFQLQLQEFINERGVLASGRPCEPGCRTFFRVCLKHFQAVVSPGPCTFGTVSTPVLGTNSFAVRDDSSGGGRNPLQLPFNFTWPGTFSLIIEAWHAPGDDLRPEALPPDALISKIAIQGSLAVGQNWLLDEQTSTLTRLRYSYRVICSDNYYGDNCSRLCKKRNDHFGHYVCQPDGNLSCLPGWTGEYCQQPICLSGCHEQNGYCSKPAECLCRPGWQGRLCNECIPHNGCRHGTCSTPWQCTCDEGWGGLFCDQDLNYCTHHSPCKNGATCSNSGQRSYTCTCRPGYTGVDCELELSECDSNPCRNGGSCKDQEDGYHCLCPPGYYGLHCEHSTLSCADSPCFNGGSCRERNQGANYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLNRGPSRMCRCRPGFTGTYCELHVSDCARNPCAHGGTCHDLENGLMCTCPAGFSGRRCEVRTSIDACASSPCFNRATCYTDLSTDTFVCNCPYGFVGSRCEFPVGLP

84-110 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. ) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.