1. Recombinant Proteins
  2. Receptor Proteins
  3. Notch family
  4. Delta-like 4 (DLL4)
  5. Delta-like protein 4/DLL4 Protein, Rhesus Macaque (HEK293, His)

Delta-like protein 4/DLL4 Protein, Rhesus Macaque (HEK293, His)

Cat. No.: HY-P76872
COA Handling Instructions

delta-like protein 4 involves in the Notch signaling pathway as Notch ligand, and activates NOTCH1 and NOTCH4. delta-like protein 4 involves in angiogenesis. Delta-like protein 4/DLL4 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived Delta-like protein 4/DLL4 protein, expressed by HEK293 , with C-His labeled tag. The total length of Delta-like protein 4/DLL4 Protein, Rhesus Macaque (HEK293, His) is 502 a.a., with molecular weight of ~56.1 KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

delta-like protein 4 involves in the Notch signaling pathway as Notch ligand, and activates NOTCH1 and NOTCH4. delta-like protein 4 involves in angiogenesis. Delta-like protein 4/DLL4 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived Delta-like protein 4/DLL4 protein, expressed by HEK293 , with C-His labeled tag. The total length of Delta-like protein 4/DLL4 Protein, Rhesus Macaque (HEK293, His) is 502 a.a., with molecular weight of ~56.1 KDa.

Background

delta-like protein 4 negatively regulates endothelial cell proliferation and migration and angiogenic sprouting.delta-like protein 4 is essential for retinal progenitor proliferation, and is required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. delta-like protein 4 inhibits V2a interneuron fate during spinal cord neurogenesis[1][2].

Biological Activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1291 μg/mL, corresponding to a specific activity is 7.746×103 units/mg.

  • Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1291 μg/mL, corresponding to a specific activity is 7.746×103 units/mg.
Species

Rhesus Macaque

Source

HEK293

Tag

C-10*His

Accession

XP_001099250 (S27-P528)

Gene ID
Molecular Construction
N-term
DLL4 (S27-P528)
Accession # XP_001099250
His
C-term
Synonyms
Delta-like protein 4; Delta4; DLL4
AA Sequence

SGVFQLQLQEFVNERGVLASGRPCEPGCRTFFRVCLKHFQAVVSPGPCTFGSVSTPVLGTNSFAVRDDSSGGGRNPLQLPFNFTWPGTFSLIIEAWHAPGDDLRPEALPPDALISKIAIQGSLAVGQNWLLDEQTSTLTRLRYSYRVICSDNYYGDNCSRLCKKRNDHFGHYVCQPDGNLSCLPGWTGEYCQQPICLSGCHEQNGYCSKPAECLCRPGWQGRLCNECIPHNGCRHGTCSTPWQCTCDEGWGGLFCDQDLNYCTHHSPCKNGATCSNSGQRSYTCTCRPGYTGVDCELELSECDSNPCRNGGSCKDQEDGYHCLCPPGYYGLHCEHSTLSCADSPCFNGGSCRERNQGASYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLNRGPSRMCRCRPGFTGTYCERHVSDCARNPCAHGGTCHDLESGLMCTCPAGFSGRRCEVRTSIDACASSPCFNRATCYTDLSTDTFVCNCPYGFVGSRCEFPVGLPPSFP

Molecular Weight

Approximately 65 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCL, 300 mM NaCl, pH 8.0, 5% Glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Delta-like protein 4/DLL4 Protein, Rhesus Macaque (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Delta-like protein 4/DLL4 Protein, Rhesus Macaque (HEK293, His)
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