DGKA Protein, Human (sf9, His, Myc)

The DGKA protein converts diacylglycerol (DAG) into phosphatidic acid (PA), regulates its levels and serves as a central switch between signaling pathways. DGKA Protein, Human (sf9, His, Myc) is the recombinant human-derived DGKA, expressed by Sf9 insect cells , with C-Myc, N-10*His labeled tag. The total length of DGKA Protein, Human (sf9, His, Myc) is 735 a.a.,

The DGKA protein functions as a diacylglycerol kinase, catalyzing the conversion of diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and intricately regulating the levels of these two bioactive lipids. This enzymatic activity positions DGKA as a central switch governing the signaling pathways activated by these second messengers, each exerting distinct effects in numerous biological processes. Additionally, DGKA plays a crucial role in the biosynthesis of complex lipids. Notably, it can efficiently phosphorylate 1-alkyl-2-acylglycerol in vitro, particularly if it contains an arachidonoyl group, showcasing its versatility. Moreover, DGKA is involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, achieved through the phosphorylation of 1-alkyl-2-acetyl glycerol. These multifaceted roles highlight DGKA's significance in lipid metabolism and its regulatory impact on diverse cellular pathways.

Human

Sf9 insect cells

C-Myc;N-10*His

P23743-1 (M1-S735)

1606

86.7 kDa

Greater than 90 % as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.