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  3. Dit Protein, Bacillus phage SPP1 (FLAG, His)

Dit Protein, Bacillus phage SPP1 (FLAG, His)

Cat. No.: HY-P701904
Handling Instructions

The Dit protein is an important component that forms a 40 Å wide channel distal to the tail in its homohexameric structure. This unique channel structure plays a crucial role in cellular processes and may facilitate DNA ejection. Dit Protein, Bacillus phage SPP1 (FLAG, His) is the recombinant Dit protein, expressed by E. coli , with N-6*His, N-Flag labeled tag. The total length of Dit Protein, Bacillus phage SPP1 (FLAG, His) is 140 a.a., .

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

The Dit protein is an important component that forms a 40 Å wide channel distal to the tail in its homohexameric structure. This unique channel structure plays a crucial role in cellular processes and may facilitate DNA ejection. Dit Protein, Bacillus phage SPP1 (FLAG, His) is the recombinant Dit protein, expressed by E. coli , with N-6*His, N-Flag labeled tag. The total length of Dit Protein, Bacillus phage SPP1 (FLAG, His) is 140 a.a., .

Background

The Dit (Distal tail protein) is a homohexameric protein that plays a crucial role in viral DNA ejection. It forms a 40 Angstroms wide channel located at the distal tip of the viral tail. Remarkably, even after the DNA ejection process, Dit remains associated with the tail structure. This implies that the protein's structural features contribute to the stability and functionality of the viral tail. The homohexameric nature of Dit suggests a coordinated and efficient mechanism, possibly aiding in the controlled release of genetic material during the viral life cycle.

Species

Others

Source

E. coli

Tag

N-6*His;N-Flag

Accession

O48459 (N2-V141)

Gene ID

955334

Molecular Construction
N-term
Flag-6*His
Dit (N2-V141)
Accession # O48459
C-term
Synonyms
Distal tail protein; Dit; Gene product 19.1; Gp19.1
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Dit Protein, Bacillus phage SPP1 (FLAG, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Dit Protein, Bacillus phage SPP1 (FLAG, His)
Cat. No.:
HY-P701904
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