DNA helicase II/uvrD Protein, E.coli

DNA helicase II/uvrD Protein, E.coli encodes an 82-kDa protein with both ATPase and helicase activities. DNA helicase II/uvrD Protein, E.coli unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. DNA helicase II/uvrD Protein, E.coli is involved in methyl-directed mismatch repair and nucleotide excision repair of DNA^[1][2][3] DNA helicase II/uvrD Protein, E.coli is the recombinant E. coli-derived DNA helicase II/uvrD protein, expressed by E. coli , with tag free. The total length of DNA helicase II/uvrD Protein, E.coli is 720 a.a., with molecular weight of ~82.0 kDa.

DNA helicase II/uvrD Protein can also initiate unwinding from a nick or blunt end at higher enzyme concentrations. The involvement of DNA helicase II/uvrD Protein, in the postincision events of nucleotide excision repair and methyl-directed mismatch repair^[1].
Mutants in DNA helicase II/uvrD Protein are strongly sensitive to UV and Azidothymidine (AZT; HY-17413), modestly sensitive to CPX and sensitivities are enhanced by addition of a mutation in radA. Introduction of a mutation in recF almost entirely suppresses the AZT sensitivity and partially suppresses the UV and CPX sensitivity of uvrD and uvrD radA mutants^[2].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

Tag Free

P03018 (M1-V720)

948347  [NCBI]

MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV

Approximately 82.0 kDa

• 
  Greater than 91% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.