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DNAJC30 Protein, Human (His)

Cat. No.: HY-P76873
COA Handling Instructions

DNAJC30 is a neuron-enriched mitochondrial protein that plays a critical regulatory role in mitochondrial respiration. It binds to the ATP synthase complex and promotes ATP synthesis. DNAJC30 Protein, Human (His) is the recombinant human-derived DNAJC30 protein, expressed by E. coli , with N-His labeled tag. The total length of DNAJC30 Protein, Human (His) is 86 a.a., with molecular weight of ~14 kDa.

For research use only. We do not sell to patients.

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Description

DNAJC30 is a neuron-enriched mitochondrial protein that plays a critical regulatory role in mitochondrial respiration. It binds to the ATP synthase complex and promotes ATP synthesis. DNAJC30 Protein, Human (His) is the recombinant human-derived DNAJC30 protein, expressed by E. coli , with N-His labeled tag. The total length of DNAJC30 Protein, Human (His) is 86 a.a., with molecular weight of ~14 kDa.

Background

DNAJC30, a mitochondrial protein enriched in neurons, assumes a crucial role as a regulator of mitochondrial respiration. This protein associates with the ATP synthase complex, playing a facilitative role in ATP synthesis. Additionally, it acts as a potential chaperone involved in the turnover of the subunits of mitochondrial complex I N-module, contributing to the degradation of N-module subunits damaged by oxidative stress and thereby enhancing the functional efficiency of complex I. Notably, DNAJC30's interaction with MT-ATP6 and ATP5MC2, both direct, underscores its involvement in these mitochondrial processes, shedding light on its significance in maintaining mitochondrial function and cellular homeostasis.

Species

Human

Source

E. coli

Tag

N-His

Accession

Q96LL9 (S39-G124)

Gene ID
Molecular Construction
N-term
His
DNAJC30 (S39-G124)
Accession # Q96LL9
C-term
Synonyms
DnaJ homolog subfamily C member 30; WBSCR18
AA Sequence

SQGDCSYSRTALYDLLGVPSTATQAQIKAAYYRQCFLYHPDRNSGSAEAAERFTRISQAYVVLGSATLRRKYDRGLLSDEDLRGPG

Molecular Weight

Approximately 14 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

DNAJC30 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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DNAJC30 Protein, Human (His)
Cat. No.:
HY-P76873
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