2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. DOT1L Protein, Human

DOT1L Protein, Human

Cat. No.: HY-P75283
Handling Instructions

The DOT1L protein, a histone methyltransferase, methylates lysine-79 of histone H3. It shows high activity against nucleosomes but not free core histones. It is broadly expressed, particularly in tissues like the testis, bone marrow, and 22 other tissues. DOT1L Protein, Human is the recombinant human-derived DOT1L protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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  • Biological Activity

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Description

The DOT1L protein, a histone methyltransferase, methylates lysine-79 of histone H3. It shows high activity against nucleosomes but not free core histones. It is broadly expressed, particularly in tissues like the testis, bone marrow, and 22 other tissues. DOT1L Protein, Human is the recombinant human-derived DOT1L protein, expressed by E. coli , with tag free.
Background

The DOT1L protein, a histone methyltransferase, is responsible for methylating lysine-79 of histone H3. While it does not exhibit significant activity against free core histones, it demonstrates substantial histone methyltransferase activity against nucleosomes. This protein is broadly expressed, with notable levels found in tissues such as the testis, bone marrow, and 22 other tissues.
Species

Human
Source

E. coli
Tag

Tag Free
Accession

Q8TEK3-1/NP_115871.1 (G2-K416)
Gene ID
Molecular Construction
N-term
DOT1L (G2-K416)
Accession # Q8TEK3-1/NP_115871.1
C-term
Synonyms
Histone H3-K79 methyltransferase; H3-K79-HMTase; DOT1L; KIAA1814; KMT4
Molecular Weight

Approximately 50 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

DOT1L Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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