1. Recombinant Proteins
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Transferases (EC 2) Ubiquitin Enzymes
  4. E3 Ligases
  5. DTX3L Protein, Human

DTX3L Protein, Human

Cat. No.: HY-P702072
Handling Instructions

DTX3L protein is an E3 ubiquitin protein ligase that cooperates with PARP9 in DNA damage repair and antiviral responses. It monoubiquitylates histones (H2A, H2B, H3, H4), especially “Lys-91” of H4, possibly signaling additional modifications. DTX3L Protein, Human is the recombinant human-derived DTX3L protein, expressed by E. coli , with tag free. The total length of DTX3L Protein, Human is 739 a.a., .

For research use only. We do not sell to patients.

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Description

DTX3L protein is an E3 ubiquitin protein ligase that cooperates with PARP9 in DNA damage repair and antiviral responses. It monoubiquitylates histones (H2A, H2B, H3, H4), especially “Lys-91” of H4, possibly signaling additional modifications. DTX3L Protein, Human is the recombinant human-derived DTX3L protein, expressed by E. coli , with tag free. The total length of DTX3L Protein, Human is 739 a.a., .

Background

DTX3L Protein, an E3 ubiquitin-protein ligase, collaborates with ADP-ribosyltransferase PARP9 to fulfill key roles in DNA damage repair and interferon-mediated antiviral responses. It monoubiquitinates various histones, including H2A, H2B, H3, and H4, particularly at 'Lys-91' of histone H4 in response to DNA damage. The exact function of H4K91ub1 remains unclear but may serve as a licensing signal for additional histone H4 post-translational modifications. The PARP1-dependent PARP9-DTX3L-mediated ubiquitination facilitates the specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. Additionally, DTX3L positively regulates STAT1-dependent interferon-stimulated gene transcription by monoubiquitinating histone H2B, promoting chromatin remodeling. Independently of its catalytic activity, DTX3L plays a crucial role in the sorting of CXCR4 from early endosomes to lysosomes, reducing E3 ligase ITCH activity and facilitating the ubiquitination of endosomal sorting complex required for transport (ESCRT-0) components HGS and STAM. Furthermore, in association with PARP9, DTX3L contributes to antiviral responses by mediating 'Lys-48'-linked ubiquitination of encephalomyocarditis virus (EMCV) and human rhinovirus (HRV) C3 proteases, leading to their proteasomal-mediated degradation. These diverse functions underscore the significance of DTX3L in regulating critical cellular processes.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

Q8TDB6 (A2-E740)

Gene ID

/

Molecular Construction
N-term
DTX3L (A2-E740)
Accession # Q8TDB6
C-term
Synonyms
DTX3L; E3 ubiquitin-protein ligase DTX3L; B-lymphoma- and BAL-associated protein; Protein deltex-3-like; RING-type E3 ubiquitin transferase DTX3L; Rhysin-2; Rhysin2
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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DTX3L Protein, Human
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HY-P702072
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