2. Recombinant Proteins
  3. Viral Proteins
  4. Dengue Virus Proteins
  5. DENV E Proteins
  6. E/Envelope Protein, Dengue virus 2 (429a.a, sf9, His)

E/Envelope Protein, Dengue virus 2 (429a.a, sf9, His)

Cat. No.: HY-P74194
SDS COA Handling Instructions

E/Envelope Protein, Dengue virus 2 (429a.a, sf9, His) is the recombinant Virus-derived E/Envelope protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of E/Envelope Protein, Dengue virus 2 (429a.a, sf9, His) is 429 a.a., with molecular weight of ~49.2 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
5 μg $45 In-stock
10 μg $75 In-stock
50 μg $215 In-stock
100 μg $340 In-stock
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Description

E/Envelope Protein, Dengue virus 2 (429a.a, sf9, His) is the recombinant Virus-derived E/Envelope protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of E/Envelope Protein, Dengue virus 2 (429a.a, sf9, His) is 429 a.a., with molecular weight of ~49.2 kDa.
Species

Virus
Source

Sf9 insect cells
Tag

C-His
Accession

AAC59274 (F247-G675)
Gene ID

/
Molecular Construction
N-term
DENV2 E (F247-G675)
Accession # AAC59274
His
C-term
Synonyms
E Protein; DENV; Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E / Envelope Protein (ECD, His Tag)
Molecular Weight

Approximately 49.2 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Solution
Formulation

Supplied as a 0.2 μm filtered solution of b 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation
SDS (251 KB)

COA (220 KB)

Handling Instructions (2659 KB)

E/Envelope Protein, Dengue virus 2 (429a.a, sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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E/Envelope Protein, Dengue virus 2 (429a.a, sf9, His)
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