E-selectin/CD62E Protein, Human (Biotinylated, HEK293, His-Avi)

E-selectin/CD62E Protein, a cell-surface glycoprotein, crucially mediates immunoadhesion by interacting with SELPLG/PSGL1 for the adhesion of neutrophils to cytokine-activated endothelium. It also potentially influences capillary morphogenesis and interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope, independently of SELPLG sulfation. E-selectin/CD62E Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived E-selectin/CD62E protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of E-selectin/CD62E Protein, Human (Biotinylated, HEK293, His-Avi) is 535 a.a., with molecular weight of 80-100 kDa.

E-selectin/CD62E Protein is a cell-surface glycoprotein that plays a crucial role in immunoadhesion. It mediates the adhesion of blood neutrophils in cytokine-activated endothelium by interacting with SELPLG/PSGL1. Additionally, E-selectin/CD62E Protein may be involved in capillary morphogenesis. It interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope, and it is worth noting that SELPLG sulfation is not necessary for this interaction.

Immobilized Biotinylated Human E-Selectin at 0.5 μg/mL (100μL/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody hFc with the EC₅₀ of 7.8-9.5 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

P16581 (W22-P556)

6401  [NCBI]

80-100 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.