Ebola virus Glycoprotein/GP Protein (QDA39862, HEK293, His)

Ebola virus Glycoprotein/GP Protein is pivotal in viral entry, serving as the receptor-binding subunit.In later infection stages, GP1 down-regulates crucial host cell surface molecules, including integrins like ITGA1-6, disrupting adhesion and potentially causing blood vessel integrity disruption.GP1 interacts with host TLR4, stimulating monocyte differentiation, leading to T-lymphocyte bystander death.It down-regulates natural killer cell function, counters BST2/tetherin, cooperates with VP40 for NF-kappa-B activation, acts as an antibody decoy, and induces cytokine transcription in host macrophages and dendritic cells through TLR4 activation.Ebola virus Glycoprotein/GP Protein (QDA39862, HEK293, His) is the recombinant Virus-derived Ebola virus Glycoprotein/GP protein, expressed by HEK293 , with C-His labeled tag.

The trimeric GP1,2 complexes of the Ebola virus Glycoprotein (GP) play a pivotal role in viral entry processes, where GP1 serves as the receptor-binding subunit and GP2 acts as the membrane fusion subunit. In the later stages of infection, GP1 down-regulates the expression of crucial host cell surface molecules involved in immune surveillance and cell adhesion. This includes the modulation of integrins such as ITGA1, ITGA2, ITGA3, ITGA4, ITGA5, ITGA6, ITGAV, and ITGB1, potentially leading to cell detachment and contributing to the disruption of blood vessel integrity, resulting in hemorrhages during infection (cytotoxicity). Additionally, GP1 interacts with host TLR4, stimulating the differentiation and activation of monocytes, leading to bystander death of T-lymphocytes. It further down-regulates the function of host natural killer cells and counteracts the antiviral effect of host BST2/tetherin, which restricts the release of progeny virions from infected cells. Interestingly, GP1 cooperates with VP40 and host BST2 to activate the canonical NF-kappa-B pathway in a manner dependent on neddylation. Furthermore, GP1 functions as a decoy for anti-GP1,2 antibodies, contributing to viral immune evasion, and interacts with host macrophages and dendritic cells, inducing the up-regulation of cytokine transcription, with this effect mediated through the activation of host TLR4^[1][2][3][4].

Virus

HEK293

C-His

QDA39862 (M1-Q650)

/

92-120&23-25 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.