1. Recombinant Proteins
  2. Viral Proteins
  3. Ebola Virus Proteins
  4. Ebola Virus GP1
  5. Ebola virus Glycoprotein/GP1 Protein (AHX24649, HEK293, His)

Ebola virus Glycoprotein/GP1 Protein (AHX24649, HEK293, His)

Cat. No.: HY-P75721
Handling Instructions

Ebola virus glycoproteins (GPs) play a key role in the virus entry process, with GP1 serving as the receptor-binding subunit and GP2 for membrane fusion.GP downregulates host cell surface molecules, disrupts adhesion and may lead to disruption of vascular integrity.Ebola virus Glycoprotein/GP1 Protein (AHX24649, HEK293, His) is the recombinant Virus-derived Ebola virus Glycoprotein/GP1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

Ebola virus glycoproteins (GPs) play a key role in the virus entry process, with GP1 serving as the receptor-binding subunit and GP2 for membrane fusion.GP downregulates host cell surface molecules, disrupts adhesion and may lead to disruption of vascular integrity.Ebola virus Glycoprotein/GP1 Protein (AHX24649, HEK293, His) is the recombinant Virus-derived Ebola virus Glycoprotein/GP1 protein, expressed by HEK293 , with C-His labeled tag.

Background

The trimeric GP1,2 complexes of the Ebola virus Glycoprotein (GP) play a crucial role in viral entry processes, where GP1 serves as the receptor-binding subunit and GP2 acts as the membrane fusion subunit. During later stages of infection, GP down-regulates the expression of various host cell surface molecules, including integrins such as ITGA1, ITGA2, ITGA3, ITGA4, ITGA5, ITGA6, ITGAV, and ITGB1, disrupting cell adhesion and contributing to the detachment of cells, potentially leading to blood vessel integrity disruption and hemorrhages. GP also interacts with host TLR4, stimulating the differentiation and activation of monocytes, resulting in bystander death of T-lymphocytes. Additionally, GP counteracts the antiviral effect of host BST2/tetherin, cooperates with VP40 and host BST2 to activate the canonical NF-kappa-B pathway, and functions as a decoy for anti-GP1,2 antibodies, contributing to viral immune evasion. Moreover, GP interacts with and activates host macrophages and dendritic cells, inducing the up-regulation of cytokine transcription through the activation of host TLR4.

Species

Virus

Source

HEK293

Tag

C-His

Accession

AHX24649 (M1-R501)

Gene ID

/

Molecular Construction
N-term
EBOV GP1 (M1-R501)
Accession # AHX24649
His
C-term
Synonyms
Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) GP1 / Glycoprotein Protein (His)
Molecular Weight

Approximately 52.3 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Ebola virus Glycoprotein/GP1 Protein (AHX24649, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Ebola virus Glycoprotein/GP1 Protein (AHX24649, HEK293, His)
Cat. No.:
HY-P75721
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