EGFR Protein, Human (621a.a, CHO, His)

EGFR Protein, Human (CHO, His) is a transmembrane protein with His tag. EGFR Protein exerts tyrosine kinase activity upon ligand induced activation.

EGF Receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. EGFR can be activated by binding EGF or at least six other structurally related protein ligands, including TGFα, HB-EGF, Betacellulin (BTC), Amphiregulin, Epiregulin, and Epigen. Upon activation, EGFR initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and, ultimately, cell proliferation. EGFR signaling plays a role in the growth and differentiation of normal cells, but elevated EGFR activity is correlated with the development and pathogenesis of certain cancers^[1].

1. The ED₅₀ is <1 μg/mL as measured in a cell proliferation assay by 3T3 cells in the presence of 25 pg/ml human EGF.
2. Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Anti- EGFR antibody, the ED₅₀ of human EGFR protein is 13.1 ng/mL, corresponding to a specific activity is 7.63×10^4 units/mg.

• 
  Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Anti- EGFR antibody, the ED₅₀ of human EGFR protein is 13.1 ng/mL, corresponding to a specific activity is 7.63×10⁴ units/mg.

Human

CHO

C-His

P00533-1 (L25-S645)

1956  [NCBI]

LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSHHHHHH

95-115 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Scaltriti M, et al. The epidermal growth factor receptor pathway: a model for targeted therapy. Clin Cancer Res. 2006 Sep 15;12(18):5268-72.  [Content Brief]