ENPP-1 Protein, Human (Biotinylated, HEK293, His-Avi)

ENPP-1 protein is a nucleotide pyrophosphatase that inhibits excessive hydroxyapatite crystal growth by preferentially hydrolyzing ATP and other nucleoside triphosphates to generate PPi, thereby critically regulating bone mineralization and soft tissue calcification. ENPP-1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived ENPP-1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of ENPP-1 Protein, Human (Biotinylated, HEK293, His-Avi) is 828 a.a., with molecular weight of 110-120 kDa.

ENPP-1 Protein, a nucleotide pyrophosphatase, plays a crucial role in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. By preferentially hydrolyzing ATP and other nucleoside 5' triphosphates, such as GTP, CTP, and UTP, ENPP-1 generates diphosphate (PPi), which inhibits the further growth of nascent hydroxyapatite crystals, thereby preventing excessive bone mineralization and calcification of soft tissues. Additionally, it participates in the hydrolysis of diadenosine polyphosphates and 3',5'-cAMP to AMP, and may regulate nucleotide sugar availability in the endoplasmic reticulum and Golgi, influencing purinergic signaling. ENPP-1 also contributes to the repression of hedgehog signaling, inhibiting ectopic joint calcification and maintaining articular chondrocytes. Furthermore, it is implicated in modulating insulin sensitivity and function, participating in melanogenesis, and can hydrolyze 2',3'-cGAMP, a second messenger involved in triggering type-I interferon production. The exact role of 2',3'-cGAMP hydrolysis in the extracellular space remains unclear.

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Immobilized Biotinylated Human ENPP-1, His Tag at 0.5 μg/mL (100 μl/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC₅₀ of 4.5 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

P22413 (K98-D925)

5167  [NCBI]

110-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in 20 mM Tris,150 mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.