ENPP-1 Protein, Mouse (HEK293, His)

ENPP-1 is a nucleotide pyrophosphatase that critically regulates bone mineralization and soft tissue calcification through the production of diphosphate (PPi).This PPi inhibits the growth of hydroxyapatite crystals and prevents unwanted mineralization.ENPP-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-1 protein, expressed by HEK293 , with C-His labeled tag.

The ENPP-1 protein, a nucleotide pyrophosphatase, plays a crucial role in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. It generates diphosphate (PPi), which inhibits the mineralization of bone and calcification of soft tissues by binding to nascent hydroxyapatite crystals, preventing their further growth. ENPP-1 exhibits preferential hydrolysis of ATP but can also hydrolyze other nucleoside 5' triphosphates and diadenosine polyphosphates. Additionally, it participates in the regulation of nucleotide sugars in the endoplasmic reticulum and Golgi, impacting purinergic signaling. The protein inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling, although the direct or indirect nature of hedgehog inhibition remains unclear. ENPP-1 is implicated in modulating insulin sensitivity and melanogenesis. Notably, it can hydrolyze 2',3'-cGAMP, a second messenger activating TMEM173/STING and triggering type-I interferon production, though the precise role of this hydrolysis in the extracellular space is not fully understood. However, ENPP-1 is unable to hydrolyze the 2',3'-cGAMP linkage isomer 3',3'-cGAMP.

Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is >100000 pmol/min/μg, as measured under the described conditions.

Mouse

HEK293

C-His

P06802-1 (K80-D906)

18605  [NCBI]

KPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKRLSRFVCSCADDCKTHNDCCINYSSVCQDKKSWVEETCESIDTPECPAEFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKNMRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSHERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDVDDIYHMTVPYGRPRILLKQHHVCLLQQQQFLTGYSLDLLMPLWASYTFLRNDQFSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPHRPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED

100-110 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.