1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Phosphatase
  4. ENPP-3
  5. ENPP-3 Protein, Rhesus Macaque (sf9, His)

ENPP-3 Protein, Rhesus Macaque (sf9, His)

Cat. No.: HY-P77357
Handling Instructions

ENPP-3 is a hydrolase that metabolizes extracellular nucleotides (ATP, GTP, UTP, CTP), which is critical for immune regulation of inflammation and chronic allergic reactions. By eliminating extracellular ATP, ENPP-3 inhibits basophil and mast cell activation and suppresses the release of inflammatory cytokines. ENPP-3 Protein, Rhesus Macaque (sf9, His) is the recombinant Rhesus Macaque-derived ENPP-3 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of ENPP-3 Protein, Rhesus Macaque (sf9, His) is 829 a.a., with molecular weight of ~97.2 KDa.

For research use only. We do not sell to patients.

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Description

ENPP-3 is a hydrolase that metabolizes extracellular nucleotides (ATP, GTP, UTP, CTP), which is critical for immune regulation of inflammation and chronic allergic reactions. By eliminating extracellular ATP, ENPP-3 inhibits basophil and mast cell activation and suppresses the release of inflammatory cytokines. ENPP-3 Protein, Rhesus Macaque (sf9, His) is the recombinant Rhesus Macaque-derived ENPP-3 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of ENPP-3 Protein, Rhesus Macaque (sf9, His) is 829 a.a., with molecular weight of ~97.2 KDa.

Background

ENPP-3, a hydrolase, exhibits the ability to metabolize various extracellular nucleotides, including ATP, GTP, UTP, and CTP. This enzymatic activity plays a crucial role in modulating immune responses, particularly during inflammation and the chronic phases of allergic reactions. ENPP-3 acts by eliminating extracellular ATP, a signaling molecule that activates basophils and mast cells, leading to the release of inflammatory cytokines. Moreover, in the lumen of the small intestine, ENPP-3 metabolizes extracellular ATP, thereby preventing ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. Besides its involvement in nucleotide metabolism, ENPP-3 also exhibits alkaline phosphodiesterase activity, adding to its multifaceted role in cellular processes.

Species

Rhesus Macaque

Source

Sf9 insect cells

Tag

N-His

Accession

XP_001103528 (R46-I874)

Gene ID
Molecular Construction
N-term
His
ENPP-3 (R46-I874)
Accession # XP_001103528
C-term
Synonyms
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3; NPP3; PD-Ibeta; PDNP3
Molecular Weight

Approximately 97.2 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

ENPP-3 Protein, Rhesus Macaque (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ENPP-3 Protein, Rhesus Macaque (sf9, His)
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