EphA4 Protein, Mouse (HEK293, Fc)

The EphA4 protein is a receptor tyrosine kinase that participates in contact-dependent bidirectional signaling with ephrin A and ephrin B ligands. EphA4 is activated by GPI-anchored EFNA and transmembrane EFNB and regulates cell morphology, integrin-dependent adhesion, and GTPase activity. EphA4 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived EphA4 protein, expressed by HEK293 , with C-hFc labeled tag.

The EphA4 protein, a receptor tyrosine kinase, engages in contact-dependent bidirectional signaling with membrane-bound ephrin family ligands on adjacent cells. Distinguished by its high promiscuity, EphA4 uniquely binds and is physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands, including EFNA1 and EFNB3. Upon activation by ephrin ligands, EphA4 modulates cell morphology and integrin-dependent cell adhesion through the regulation of Rac, Rap, and Rho GTPases activity. Crucial in the development of the nervous system, EphA4 controls various steps of axonal guidance, including the establishment of corticospinal projections and the segregation of motor and sensory axons during neuromuscular circuit development. In synaptic plasticity, EphA4 participates by phosphorylating CDK5 at 'Tyr-15,' leading to the regulation of RHOA and dendritic spine morphogenesis. Furthermore, EphA4 plays roles in repair after injury by preventing axonal regeneration and in angiogenesis, contributing to central nervous system vascular formation. Its promiscuity extends its involvement in various cell-cell signaling processes, regulating the development of the thymic epithelium and, during the development of the cochlear organ of Corti, facilitating pillar cell separation through the formation of a ternary complex with ADAM10 and CADH1, leading to the cleavage of CADH1 and disruption of adherens junctions. EphA4 also phosphorylates CAPRIN1, promoting CAPRIN1-dependent formation of a membraneless compartment.

Measured by its binding ability in a functional ELISA . Immobilized mouse EPHA5 at 2 μg/mL (100 μl/well) can bind mouse EFNA4-Fc with a linear range of 1.28-32 ng/mL.

Mouse

HEK293

C-hFc

Q03137-1 (V20-T547)

13838  [NCBI]

MAGIFYFILFSFLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEASQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRESQFGKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEEQNGECQACKIGYYKALSTDASCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTRVSITDLLAHTNYTFEIWAVNGVSKYNPSPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANST

Approximately 110 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.