1. Recombinant Proteins
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Eph Receptors
  5. EphA5
  6. EPHA5 Protein, Cynomolgus (HEK293, His)

EPHA5 Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P700715
SDS COA Handling Instructions

EPHA5 is a member of the erythropoietin-producing human liver cancer receptor (Eph receptor) that regulates embryogenesis by fine-tuning cell adhesion, localization, and migration, particularly in the nervous system. EPHA5 enhances the invasion and migration of esophageal squamous cell carcinoma through epithelial-mesenchymal transformation by activating the Wnt/β-catenin pathway. EPHA5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived EPHA5 protein, expressed by HEK293 , with C-His labeled tag. The total length of EPHA5 Protein, Cynomolgus (HEK293, His) is 548 a.a., with molecular weight of 70-75 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $220 In-stock
50 μg $420 In-stock
100 μg $670 In-stock
> 100 μg   Get quote  

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

EPHA5 is a member of the erythropoietin-producing human liver cancer receptor (Eph receptor) that regulates embryogenesis by fine-tuning cell adhesion, localization, and migration, particularly in the nervous system. EPHA5 enhances the invasion and migration of esophageal squamous cell carcinoma through epithelial-mesenchymal transformation by activating the Wnt/β-catenin pathway. EPHA5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived EPHA5 protein, expressed by HEK293 , with C-His labeled tag. The total length of EPHA5 Protein, Cynomolgus (HEK293, His) is 548 a.a., with molecular weight of 70-75 kDa.

Background

Erythropoietin-producing human liver cancer receptors (Eph receptors) are the largest subgroup of receptor tyrosine kinases. Eph receptors were originally found to regulate embryogenesis by fine-tuning cell adhesion, localization, and migration, particularly in the nervous system. EPHA5 is a member of the Eph receptor and is involved in a variety of biological activities, including tumorigenesis and the progression of different cancers. EPHA5 enhances the invasion and migration of esophageal squamous cell carcinoma through epithelial-mesenchymal transformation by activating the Wnt/β-catenin pathway[1][2][3].

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Cynomolgus

Source

HEK293

Tag

C-His

Accession

A0A2K5W6J6 (P25-Q572)

Gene ID
Molecular Construction
N-term
EPHA5 (P25-Q572)
Accession # A0A2K5W6J6
His
C-term
Synonyms
EHK-1; EK7; BSK; EHK1; HEK7; TYRO4; EphA5; Rek7; TYRO4HEK7CEK7
Molecular Weight

70-75kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
EPHA5 Protein, Cynomolgus (HEK293, His)
Cat. No.:
HY-P700715
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