EphB1 Protein, Rhesus Macaque (HEK293, His)

EphB1, a tyrosine kinase receptor, was promiscuously bound to the transmembrane ligand ephrin-B family on adjacent cells, initiating contact-dependent bidirectional signaling. EphB1 activates the MAPK/ERK and JNK signaling cascades that regulate cell migration and adhesion, respectively. EphB1 promotes differentiation and maturation of dendritic cells in non-small cell lung cancer. EphB1 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived EphB1 protein, expressed by HEK293 , with C-His labeled tag.

EphB1, a tyrosine kinase receptor, was promiscuously bound to the transmembrane ligand ephrin-B family on adjacent cells, initiating contact-dependent bidirectional signaling. The downstream signaling pathway originating from the receptor is called the forward signaling pathway, while the downstream signaling pathway for ephrin ligands is called the reverse signaling pathway. The homologous/functional ligands of this receptor include EFNB1, EFNB2 and EFNB3. In nervous system development, EphB1 regulates retinal axon guidance by redirecting the axons of ventral temporal retinal ganglion cells on the ipsilateral midline of the optic crossover, possibly through repulsive interactions with EFNB2. In the adult nervous system, EphB1, together with EFNB3, controls the chemotaxis, proliferation, and polarity of hippocampal neural progenitors. In addition to axonal guidance, EphB1 also plays a crucial redundant role with other ephrin-B receptors in the development and maturation of dendritic spines and the formation of synapses. EphB1 may regulate angiogenesis and play a role in targeting cell migration and adhesion. After being activated by EFNB1 and other possible ephrin-B ligands, EphB1 activated the MAPK/ERK and JNK signaling cascades, regulating cell migration and adhesion, respectively. EphB1 is involved in maintaining the pool of satellite cells (muscle stem cells) by promoting self-renewal of satellite cells (muscle stem cells) and reducing their activation and differentiation. EphB1 promotes differentiation and maturation of dendritic cells in non-small cell lung cancer^[1][2][3][4].

Measured by its binding ability in a functional ELISA. Immobilized Human EFNB1 at 2 μg/ml (100 μl/well) can bind Rhesus Macaque EPHB1. The ED₅₀ for this effect is 14.49 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human EFNB1 at 2 μg/ml (100 μL/well) can bind Rhesus Macaque EPHB1. The ED₅₀ for this effect is 14.49 ng/mL.

Rhesus Macaque

HEK293

C-10*His

XP_001115263.1 (M18-P540)

717211  [NCBI]

MEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLP

Approximately 58-77 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.