EphB3 Protein, Rat (HEK293, His)

The EphB3 protein is a receptor tyrosine kinase that binds to the transmembrane ephrin B ligand to initiate bidirectional signaling. The forward signal originates from the receptor, while the reverse signal originates from the ephrin ligand. EphB3 Protein, Rat (HEK293, His) is the recombinant rat-derived EphB3 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphB3 Protein, Rat (HEK293, His) is 508 a.a., with molecular weight of ~60-70 kDa.

The EphB3 protein, a receptor tyrosine kinase, engages in promiscuous binding to transmembrane ephrin-B family ligands on adjacent cells, initiating contact-dependent bidirectional signaling. The downstream pathway originating from the receptor is known as forward signaling, while the signaling pathway downstream of the ephrin ligand is termed reverse signaling. EphB3 generally exhibits an overlapping and redundant function with EPHB2, particularly in axon guidance during development, regulating neurons forming the corpus callosum and the anterior commissure—two major interhemispheric connections between the temporal lobes of the cerebral cortex. Alongside its role in axon guidance, EphB3 plays a crucial redundant role with other ephrin-B receptors in the development and maturation of dendritic spines and the formation of excitatory synapses. The protein also governs various aspects of development, including angiogenesis, palate development, and thymic epithelium development. Through both forward and reverse signaling within the EFNB2/EPHB3 complex, EphB3 regulates the migration and adhesion of cells involved in tubularizing the urethra and septating the cloaca. Notably, EphB3 plays a significant role in intestinal epithelium differentiation by segregating progenitor cells from differentiated cells in the crypt.

Immobilized mouse EPHB3 10 μg/mL (100 μL/well) can bind mouse EFNB1, The ED₅₀ for this effect is 8.187 ng/mL.

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  Immobilized mouse EPHB3 10 μg/mL (100 μL/well) can bind mouse EFNB1, The ED₅₀ for this effect is 8.187 ng/mL.

Rat

HEK293

C-His

D3ZH39 (L30-T537)

287989  [NCBI]

LEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRREVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIANAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKETQCRACPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDTADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCRGSSGAGGPATCSRCDDNVEFEPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLHLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSKGIASTVTSQKNSVQLDGLQPDARYVVQVRARTVAGYGQYSRPAEFET

Approximately 60-70 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.