Ephrin-B1/EFNB1 Protein, Human (HEK293, His)

Ephrin-B1/EFNB1 Protein, a transmembrane ligand, interacts with Eph receptors, facilitating bidirectional signaling. It binds EPHB1/ELK and EPHB2/3, inducing axon collapse and growth cone orientation. EFNB1 also interacts with GRIP1/2, TLE1, and enhances ZHX2's transcriptional repression activity. These interactions highlight EFNB1's role in diverse developmental processes. Ephrin-B1/EFNB1 Protein, Human (HEK293, His) is the recombinant human-derived Ephrin-B1/EFNB1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Ephrin-B1/EFNB1 Protein, Human (HEK293, His) is 205 a.a., with molecular weight of ~34.0 kDa.

Ephrin-B1/EFNB1 Protein is a cell surface transmembrane ligand that interacts with Eph receptors, a family of receptor tyrosine kinases, crucial for neuronal, vascular, and epithelial development. This binding leads to contact-dependent bidirectional signaling between neighboring cells. Ephrin-B1/EFNB1 shows high affinity for the receptor tyrosine kinase EPHB1/ELK and can also bind EPHB2 and EPHB3. In vitro studies demonstrate that it can induce the collapse of commissural axons and growth cones, suggesting a role in orienting longitudinally projecting axons. Ephrin-B1/EFNB1 interacts with GRIP1 and GRIP2 through its PDZ-binding motif and with TLE1. Additionally, the intracellular domain peptide of Ephrin-B1/EFNB1 interacts with ZHX2, enhancing ZHX2's transcriptional repression activity.

Human

HEK293

C-6*His

P98172 (L28-G232)

1947  [NCBI]

LAKNLEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAGRPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNRPEQEIRFTIKFQEFSPNYMGLEFKKHHDYYITSTSNGSLEGLENREGGVCRTRTMKIIMKVGQDPNAVTPEQLTTSRPSKEADNTVKMATQAPGSRGSLGDSDGKHETVNQEEKSGPGASGGSSGDPDG

Approximately 34.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.