Epstein-Barr virus Glycoprotein gp350/GP350 Protein (HEK293, His)

The Epstein-Barr viral glycoprotein gp350 (GP350) is critical for initiating attachment of viral particles to host B lymphocytes, a critical step in viral entry. By binding to host CR2 on B lymphocytes, GP350 promotes the subsequent binding of the viral glycoprotein gp42 to HLA class II molecules. Epstein-Barr virus Glycoprotein gp350/GP350 Protein (HEK293, His) is the recombinant Virus-derived Epstein-Barr virus Glycoprotein gp350/GP350 protein, expressed by HEK293 , with C-His labeled tag. The total length of Epstein-Barr virus Glycoprotein gp350/GP350 Protein (HEK293, His) is 490 a.a., with molecular weight of ~53.7 kDa.

Epstein-Barr virus Glycoprotein gp350 (GP350) plays a pivotal role in initiating virion attachment to host B-lymphocyte cells, a crucial step in the virus entry process. GP350 achieves this by binding to host CR2 at the surface of B-lymphocytes, thereby facilitating the subsequent binding of viral glycoprotein gp42 to HLA class II molecules. This attachment event triggers the fusion of the virion with the host cell membrane, leading to the invasion of the host cell by the virus. The interaction between GP350 and host CR2 is a key molecular interaction central to the initial stages of Epstein-Barr virus infection.

Virus

HEK293

C-His

P03200 (M1-P490)

3783713  [NCBI]

Approximately 53.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping with dry ice.