ERVW-1 Protein, Human (His-SUMO)

The ERVW-1 protein is an endogenous retroviral envelope protein that retains fusogenic properties critical for trophoblast fusion and syncytium formation during placental morphogenesis. Its fusion ability involves binding to SLC1A4 and SLC1A5. ERVW-1 Protein, Human (His-SUMO) is the recombinant human-derived ERVW-1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

The endogenous retroviral envelope protein ERVW-1 has preserved its inherent fusogenic properties, playing a pivotal role in trophoblast fusion and the establishment of a syncytium during placental morphogenesis. Its capacity for inducing fusion is facilitated through the binding of SLC1A4 and SLC1A5. Endogenous envelope proteins, like ERVW-1, may exhibit varied evolutionary fates, maintaining, losing, or altering their original functions over time. Retroviral envelope proteins, comprising a surface protein (SU) for receptor recognition and a transmembrane protein (TM) acting as a class I viral fusion protein, are critical for receptor recognition and membrane fusion in early infection. ERVW-1 likely adopts three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. In the context of viral and target cell membrane fusion, the coiled coil regions (heptad repeats) transition to a trimer-of-hairpins structure, bringing the fusion peptide close to the ectodomain's C-terminal region—a structural arrangement believed to drive membrane apposition and subsequent fusion.

Human

E. coli

N-His;N-SUMO

Q9UQF0-1 (A21-Q443)

30816  [NCBI]

APPPCRCMTSSSPYQEFLWRMQRPGNIDAPSYRSLSKGTPTFTAHTHMPRNCYHSATLCMHANTHYWTGKMINPSCPGGLGVTVCWTYFTQTGMSDGGGVQDQAREKHVKEVISQLTRVHGTSSPYKGLDLSKLHETLRTHTRLVSLFNTTLTGLHEVSAQNPTNCWICLPLNFRPYVSIPVPEQWNNFSTEINTTSVLVGPLVSNLEITHTSNLTCVKFSNTTYTTNSQCIRWVTPPTQIVCLPSGIFFVCGTSAYRCLNGSSESMCFLSFLVPPMTIYTEQDLYSYVISKPRNKRVPILPFVIGAGVLGALGTGIGGITTSTQFYYKLSQELNGDMERVADSLVTLQDQLNSLAAVVLQNRRALDLLTAERGGTCLFLGEECCYYVNQSGIVTEKVKEIRDRIQRRAEELRNTGPWGLLSQ

Approximately 63.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.