Erythropoietin/EPO Protein, Mouse (HEK293, His)

Erythropoietin (EPO) is essential for erythrocyte regulation, influencing proliferation, differentiation, and maintaining a balanced erythrocyte mass. Binding to its receptor, EPOR, induces dimerization and activates JAK2, initiating signaling pathways, including STAT1 and STAT3. These events are pivotal in orchestrating erythropoiesis and maintaining red blood cell homeostasis. Erythropoietin/EPO Protein, Mouse (HEK293, His) is the recombinant mouse-derived Erythropoietin/EPO protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Erythropoietin/EPO Protein, Mouse (HEK293, His) is 166 a.a., with molecular weight of 30-40 kDa.

Erythropoietin (EPO) is a hormone crucial for regulating the proliferation and differentiation of erythrocytes, as well as maintaining a balanced circulating erythrocyte mass. Upon binding to its receptor, EPOR, EPO triggers EPOR dimerization and subsequent activation of JAK2, initiating specific downstream signaling pathways, notably involving STAT1 and STAT3. These molecular events play a pivotal role in orchestrating the processes that contribute to erythropoiesis and the overall homeostasis of red blood cell levels.

The cell proliferation assay using TF 1 human erythroleukemic cells has an ED₅₀ value of 0.05-0.35 ng/mL.

Mouse

HEK293

C-6*His

Q0VED9 (A27-R192)

13856  [NCBI]

APPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR

30-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.