Erythropoietin/EPO Protein, Mouse (HEK293)

This gene encodes the erythropoietin (EPO) protein that is critical for regulating erythropoiesis. It is mainly expressed in the liver during fetal development and is transferred to the kidneys in adults. Erythropoietin/EPO Protein, Mouse (HEK293) is the recombinant mouse-derived Erythropoietin/EPO protein, expressed by HEK293 , with tag free. The total length of Erythropoietin/EPO Protein, Mouse (HEK293) is 166 a.a., with molecular weight of ~23-36 kDa.

This gene encodes the glycoprotein hormone erythropoietin, which plays a crucial role in regulating red blood cell production and hemoglobin biosynthesis. While this gene is predominantly expressed in the liver during fetal development, it shifts to the kidneys in adults. Complete absence of the encoded protein leads to embryonic lethal anemia in mice, and conditional inactivation in adult mice results in chronic, normocytic, and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Various isoforms are generated through alternative splicing. Notably, the expression of this gene is low in the reference dataset.

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 0.2134 ng/mL, corresponding to a specific activity is 4.68×10^6 units/mg.

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  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.The ED50 for this effect is 0.2134 ng/ml.corresponding to a specific activity is 4.68×10⁶units/mg.

Mouse

HEK293

Tag Free

NP_031968.1 (A27-R192)

13856

APPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR

Approximately 23-36 kDa due to glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.