ESAM Protein, Rat (HEK293, Fc)

ESAM protein promotes aggregation through a homophilic molecular interaction and interacts with MAGI1, enhancing its role in cellular processes. ESAM Protein, Rat (HEK293, Fc) is the recombinant rat-derived ESAM protein, expressed by HEK293 , with C-hFc labeled tag. The total length of ESAM Protein, Rat (HEK293, Fc) is 222 a.a., with molecular weight of 57-70 kDa.

ESAM Protein has the ability to facilitate aggregation, primarily through a homophilic molecular interaction. It also interacts with MAGI1, thereby contributing to its functional role in cellular processes.

Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ for this effect is 40.66 ng/mL. Corresponding to a specific activity is 2.459×10⁴ Unit/mg.

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  Measured in a cell proliferation assay using MDA-MB-231 cells.The ED₅₀ for this effect is 40.66 ng/mL. Corresponding to a specific activity is 2.459×10⁴ Unit/mg.

Rat

HEK293

C-hFc

Q6AYD4 (Q30-A251)

300519  [NCBI]

QMELHVPPGLNKLEAVEGEEVVLPAWYTMAREESSSHPWEAPFLIWFLEQEGKEPKQVLSYFKGSLSNKPGVTLVHSISTRNVSLRLDALQEGDSGTYRCSVNAYDSDGKNIGHSIKTIELKVLVPPAPPSCSFQGVPYVGTNVTLNCKSPRSKPTAQYQWERLAPSSQVFFGPALDTVRGSLKLTNISTAMSGVYVCKAQNRVGFAQCNVTLDVMTGSKAA

Approximately 57-70 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.