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  3. EsxA Protein, Mycobacterium tuberculosis (His, Myc)

EsxA Protein, Mycobacterium tuberculosis (His, Myc)

Cat. No.: HY-P702796
Data Sheet Handling Instructions Technical Support

EsxA Protein, Mycobacterium tuberculosis (His, Myc) is the recombinant EsxA, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of EsxA Protein, Mycobacterium tuberculosis (His, Myc) is 94 a.a., .

For research use only. We do not sell to patients.

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Size Price Stock Quantity
Free Sample   Apply now
5 μg USD 120 In-stock
10 μg USD 195 In-stock
50 μg USD 500 In-stock
100 μg USD 800 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

EsxA Protein, Mycobacterium tuberculosis (His, Myc) is the recombinant EsxA, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of EsxA Protein, Mycobacterium tuberculosis (His, Myc) is 94 a.a., .

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mycobacterium tuberculosis EsxA is immobilized at 2.5 µg/mL(100 µL/well) can bind Anti-EsxB antibody. The ED50 for this effect is 468 ng/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Mycobacterium tuberculosis EsxA is immobilized at 2.5 µg/mL(100 µL/well) can bind Anti-EsxB antibody . The ED50 for this effect is 468 ng/mL.
Species

Others

Source

E. coli

Tag

N-10*His;C-Myc

Accession

P0A565 (T2-A95)

Gene ID

45427879

Synonyms
esxA; esaT6; BQ2027_MB39056 kDa early secretory antigenic target; ESAT-6
AA Sequence

TEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQNLARTISEAGQAMASTEGNVTGMFA

Molecular Weight

Approximately 13 kDa

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

EsxA Protein, Mycobacterium tuberculosis (His, Myc) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
EsxA Protein, Mycobacterium tuberculosis (His, Myc)
Cat. No.:
HY-P702796
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