Exfoliative toxin A Protein, S. aureus (His)

Exfoliative toxin A protein, with serine protease-like properties, binds to skin protein profilaggrin, demonstrating cleavage activity after acidic residues. Its involvement is associated with impetigous diseases, particularly staphylococcal scalded skin syndrome (SSSS). Exfoliative toxin A Protein, S. aureus (His) is the recombinant Staphylococcus aureus-derived Exfoliative toxin A protein, expressed by E. coli , with N-6*His labeled tag. The total length of Exfoliative toxin A Protein, S. aureus (His) is 242 a.a., with molecular weight of ~30.9 kDa.

The Exfoliative toxin A protein possesses serine protease-like properties and has an affinity for binding to the skin protein profilaggrin. It exhibits cleavage activity specifically after acidic residues. Notably, exfoliative toxins, including Exfoliative toxin A, are implicated in causing impetigous diseases commonly known as staphylococcal scalded skin syndrome (SSSS).

Staphylococcus aureus

E. coli

N-6*His

P09331 (E39-E280)

/

EVSAEEIKKHEEKWNKYYGVNAFNLPKELFSKVDEKDRQKYPYNTIGNVFVKGQTSATGVLIGKNTVLTNRHIAKFANGDPSKVSFRPSINTDDNGNTETPYGEYEVKEILQEPFGAGVDLALIRLKPDQNGVSLGDKISPAKIGTSNDLKDGDKLELIGYPFDHKVNQMHRSEIELTTLSRGLRYYGFTVPGNSGSGIFNSNGELVGIHSSKVSHLDREHQINYGVGIGNYVKRIINEKNE

Approximately 30.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.