FBP1 Protein, Human (HEK293, His)

The FBP1 protein plays a crucial role in cellular processes by catalyzing the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate, acting as a rate-limiting enzyme in gluconeogenesis. In addition to playing a key role in glucose metabolism, FBP1 also plays an important role in regulating glucose sensing and insulin secretion in pancreatic β cells. FBP1 Protein, Human (HEK293, His) is the recombinant human-derived FBP1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of FBP1 Protein, Human (HEK293, His) is 337 a.a., with molecular weight of 35-38 kDa.

The FBP1 protein is a key enzyme that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, playing a crucial role as a rate-limiting enzyme in gluconeogenesis. Its enzymatic activity is facilitated by divalent cations. FBP1 is integral to the regulation of glucose homeostasis and insulin secretion in pancreatic beta-cells, influencing glucose sensing. Additionally, FBP1 is implicated in the modulation of glycerol gluconeogenesis in the liver. Notably, this protein serves as a significant regulator of appetite and adiposity, as increased expression in the liver following nutrient excess leads to elevated levels of circulating satiety hormones and a reduction in appetite-stimulating neuropeptides. This suggests that FBP1 acts as a feedback mechanism to limit weight gain in response to nutritional abundance.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P09467 (A2-Q338)

2203  [NCBI]

ADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ

35-38 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 1 mM EDTA, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.