FCRN-B2M Protein, Human (HEK293, His)

FCGRT is an IgG Fc receptor. Fc receptor expression is up-regulated by pro-inflammatory cytokines TNF and down-regulated by IFN-γ. FCGRT plays a role in regulating IgG and serum albumin conversion. Overexpression of FCGRT is also associated with poor prognosis of tumors. FCRN-B2M Protein, Human (HEK293, His) is a recombinant protein dimer complex containing human-derived FCRN-B2M protein, expressed by HEK293 , with C-6*His labeled tag. FCRN-B2M Protein, Human (HEK293, His), has molecular weight of ~32 & 12 kDa, respectively.

IgG receptor FcRn large subunit p51 is an IgG Fc receptor with a molecular structure similar to MHC Class I and also binds to β-2 microglobulin. In rodents, FcRn was originally thought to be a receptor that trantranks maternal immunoglobulin G (IgG) from mother to newborn offspring via breast milk and is therefore known as a neonatal Fc receptor. FcRn has also been shown to play a role in regulating IgG and serum albumin conversion, and neonatal Fc receptor expression is up-regulated by pro-inflammatory cytokines TNF and down-regulated by IFN-γ. In the acidic endosomes of endothelial and hematopoietic cells, monomer IgG binds to FcRn, circulates IgG to the cell surface, where it is released into the circulation, regulating, in addition to IgG, homeostasis of the circulating protein albumin /ALB. The up-regulated expression of FCGRT in bladder cancer may serve as a key neutrophil gene associated with poor prognosis. FCGRT overexpression was also associated with decreased PD-L1 expression and decreased tumor mutation load (TMB) level^{[1][2][3][4][5][6]}.

Measured by its binding ability in a functional ELISA. When FCRN-B2M is immobilized at 2.00 µg/mL (100 µL/well), can bind  Biotinylated Human lgG1. The ED₅₀ for this effect is 81.59 ng/mL.

Human

HEK293

C-6*His

AAF72596 (A24-S297)&P61769 (I21-M119)

2217  [NCBI]&567  [NCBI]

A1:
AESHLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYNSLRGEAEPCGAWVWENQVSWYWEKETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELGPDNTSVPTAKFALNGEEFMNFDLKQGTWGGDWPEALAISQRWQQQDKAANKELTFLLFSCPHRLREHLERGRGNLEWKEPPSMRLKARPSSPGFSVLTCSAFSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSLTVKSGDEHHYCCIVQHAGLAQPLRVELESPAKSS
A2:
IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Approximately 32 & 12 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 50 mM HEPES,150 mM Nacl, 0.02% Tween20, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.