FDPS Protein, Mouse (His)

FDPS proteins are key enzymes in isoprenoid biosynthesis and play a crucial role in the formation of farnesyl diphosphate (FPP). FPP is a precursor of essential metabolites involved in protein farnesylation and geranylgeranylation. FDPS Protein, Mouse (His) is the recombinant mouse-derived FDPS protein, expressed by E. coli , with N-His labeled tag. The total length of FDPS Protein, Mouse (His) is 353 a.a., with molecular weight of ~42.8 kDa.

Farnesyl diphosphate synthase (FDPS) emerges as a pivotal enzyme in isoprenoid biosynthesis, driving the crucial formation of farnesyl diphosphate (FPP). FPP, a key precursor for various essential metabolites such as sterols, dolichols, carotenoids, and ubiquinones, also serves as a substrate for protein farnesylation and geranylgeranylation. The catalytic activity of FDPS involves the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, first with dimethylallyl pyrophosphate and then with the resulting geranylpyrophosphate, ultimately yielding the essential product, farnesyl pyrophosphate. This intricate enzymatic process underscores FDPS's central role in the synthesis of fundamental cellular components and post-translational modifications critical for diverse cellular functions.

Measured in a cell proliferation assay using H4 human neuroglioma cells. The ED₅₀ for this effect is 2.560 ng/mL. Corresponding to a specific activity is 3.906×10^[5] Unit/mg.

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  Measured in a cell proliferation assay using H4 human neuroglioma cells. The ED₅₀ for this effect is 2.560 ng/mL. Corresponding to a specific activity is 3.906×10⁵ Unit/mg.

Mouse

E. coli

N-His

Q920E5 (M1-K353)

110196  [NCBI]

MNGNQKLDAYNQEKQNFIQHFSQIVKVLTEKELGHPEIGDAIARLKEVLEYNALGGKYNRGLTVVQAFQELVEPKKQDAESLQRALTVGWCVELLQAFFLVSDDIMDSSLTRRGQICWYQKPGIGLDAINDALLLEASIYRLLKFYCREQPYYLNLLELFLQSSYQTEIGQTLDLMTAPQGHVDLGRYTEKRYKSIVKYKTAFYSFYLPIAAAMYMAGIDGEKEHANALKILMEMGEFFQVQDDYLDLFGDPSVTGKVGTDIQDNKCSWLVVQCLLRASPQQRQILEENYGQKDPEKVARVKALYEALDLQSAFFKYEEDSYNRLKSLIEQCSAPLPPSIFMELANKIYKRRK

Approximately 42.8 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.