FGFR-4 Protein, Rat (HEK293, C-hFc)

The FGFR-4 protein is a tyrosine-protein kinase receptor for fibroblast growth factor that regulates cellular processes, lipid metabolism, bile acid biosynthesis, and more.Its effects include downregulation of CYP7A1 in response to FGF19.FGFR-4 Protein, Rat (HEK293, C-hFc) is the recombinant rat-derived FGFR-4 protein, expressed by HEK293 , with C-hFc labeled tag.

FGFR-4 protein, a tyrosine-protein kinase, functions as a cell-surface receptor for fibroblast growth factors, playing a crucial role in the regulation of cell proliferation, differentiation, and migration. Additionally, it contributes to the control of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism, and phosphate homeostasis. Its significance is highlighted in the normal down-regulation of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Upon ligand binding, FGFR-4 activates various signaling cascades, including the phosphorylation of PLCG1 and FRS2. This activation leads to the production of cellular signaling molecules like diacylglycerol and inositol 1,4,5-trisphosphate. Furthermore, FRS2 phosphorylation triggers the recruitment of GRB2, GAB1, PIK3R1, and SOS1, activating RAS, MAPK1/ERK2, MAPK3/ERK1, the MAP kinase signaling pathway, and the AKT1 signaling pathway. Notably, FGFR-4 promotes SRC-dependent phosphorylation of the matrix protease MMP14, facilitating its lysosomal degradation. To regulate FGFR-4 signaling, the receptor undergoes internalization and degradation, a process facilitated by MMP14.

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human FGF18 at 10 μg/mL (100 μl/well) can bind Rat FGFR4 , The EC₅₀ of Rat FGFR4 is <1.17 μg/mL.
3.Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀  for this effect is 1.025 ng/mL, corresponding to a specific activity is 9.76×10^5 units/mg.

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  Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 1.025 ng/mL, corresponding to a specific activity is 9.76×105 units/mg.

Rat

HEK293

C-hFc

Q498D6 (F17-D367)

25114

FSLEASEEMEQEPCPAPISEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRLASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLIMDDSLPSINNEDPKTLSSSSSGHSYLQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKNGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSNVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPAEEEDLAWTTATSEARYTD

Approximately 110 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.