FLT3 Protein, Human (T227M, HEK293, His)

FLT3 is a tyrosine-protein kinase receptor that regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. It phosphorylates downstream effectors such as SHC1 and AKT1, activating signaling cascades involving MTOR, RAS, and MAP kinases. FLT3 Protein, Human (T227M, HEK293, His) is the recombinant human-derived FLT3 protein, expressed by HEK293 , with C-His labeled tag.

FLT3, a tyrosine-protein kinase, functions as a cell-surface receptor for the cytokine FLT3LG, exerting regulatory control over the differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. This receptor facilitates the phosphorylation of various downstream effectors, including SHC1 and AKT1, and activates signaling cascades involving MTOR, RAS, MAPK1/ERK2, and/or MAPK3/ERK1. Moreover, it plays a pivotal role in the phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. While wild-type FLT3 activation leads to modest STAT5A or STAT5B activation, mutations causing constitutive kinase activity result in heightened cell proliferation and resistance to apoptosis, underscoring its role in fostering aberrant signaling pathways.

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Flt-3 Ligand at 10 μg/mL can bind Human Flt-3. The ED₅₀ for this effect is 0.3422 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Flt-3 Ligand at 10 μg/mL can bind Human Flt-3 .The ED₅₀ for this effect is 0.3422 μg/mL.

Human

HEK293

C-10*His

P36888-1 (N27-N541, T227M)

2322  [NCBI]

NQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGCALRPQSSGTVYEAAAVEVDVSASITLQVLVDAPGNISCLWVFKHSSLNCQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNTLLYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESPAVVKKEEKVLHELFGMDIRCCARNELGRECTRLFTIDLNQTPQTTLPQLFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEMSTYSTNRTMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSEDYEIDQYEEFCFSVRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISKFCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRKPQVLAEASASQASCFSDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNMSEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDN

Approximately 80-110 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.