1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. fum Protein, Corynebacterium glutamicum (His)

fum Protein, Corynebacterium glutamicum (His)

Cat. No.: HY-P702122
Handling Instructions

Fum proteins crucially catalyze the interconversion of fumarate to L-malate in the tricarboxylic acid (TCA) cycle, maintaining metabolite flow in cellular energy metabolism. This critical step, if precisely regulated, helps generate energy and generates intermediates critical to cellular processes. fum Protein, Corynebacterium glutamicum (His) is the recombinant fum protein, expressed by E. coli , with N-6*His labeled tag. The total length of fum Protein, Corynebacterium glutamicum (His) is 469 a.a., .

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

Fum proteins crucially catalyze the interconversion of fumarate to L-malate in the tricarboxylic acid (TCA) cycle, maintaining metabolite flow in cellular energy metabolism. This critical step, if precisely regulated, helps generate energy and generates intermediates critical to cellular processes. fum Protein, Corynebacterium glutamicum (His) is the recombinant fum protein, expressed by E. coli , with N-6*His labeled tag. The total length of fum Protein, Corynebacterium glutamicum (His) is 469 a.a., .

Background

The fum Protein plays a crucial role in the tricarboxylic acid (TCA) cycle, where it catalyzes the stereospecific interconversion of fumarate to L-malate. This enzymatic activity is pivotal for maintaining the flow of metabolites within the TCA cycle, a central pathway in cellular energy metabolism. The conversion of fumarate to L-malate by fum is a key step that contributes to the production of energy and the generation of intermediates essential for various cellular processes. The precise regulation of this enzymatic reaction highlights the significance of fum in orchestrating metabolic flux and energy production within the cell.

Species

Others

Source

E. coli

Tag

N-6*His

Accession

Q8NRN8 (M1-F469)

Gene ID

/

Molecular Construction
N-term
6*His
fum (M1-F469)
Accession # Q8NRN8
C-term
Synonyms
fumC; Fumarate hydratase class II; Fumarase C; Aerobic fumarase; Iron-independent fumarase
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

fum Protein, Corynebacterium glutamicum (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
fum Protein, Corynebacterium glutamicum (His)
Cat. No.:
HY-P702122
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