Furin/PCSK3 Protein, Human (HEK293, His)

Furin/PCSK3 protein is a ubiquitous endoprotease in the constitutive secretory pathway that efficiently cleaves the RX(K/R)R consensus motif. Its versatility is evident in processing TGFB1 (essential for TGF-β-1 activation) and converting the brain natriuretic factor prohormone into active BNP(1-32). Furin/PCSK3 Protein, Human (HEK293, His) is the recombinant human-derived Furin/PCSK3 protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of Furin/PCSK3 Protein, Human (HEK293, His) is 548 a.a., with molecular weight of 60-75 kDa.

Furin/PCSK3 protein is a ubiquitous endoprotease found within constitutive secretory pathways, exhibiting a remarkable ability to cleave at the RX(K/R)R consensus motif. This enzymatic versatility is evidenced by its involvement in the processing of TGFB1, a crucial step in the activation of TGF-beta-1. Furin/PCSK3 also plays a pivotal role in the conversion of the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32). Furthermore, by mediating the processing of the accessory subunit ATP6AP1/Ac45 of the V-ATPase, Furin/PCSK3 contributes to the regulation of acidic environments within dense-core secretory granules in islets of Langerhans cells. Notably, in the context of microbial infection, this protease is implicated in the cleavage and activation of diphtheria toxin DT.

Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC. The specific activity is 191.99 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-His;C-6*His

P09958 (Q27-A574)

5045  [NCBI]

QKVFTNTWAVRIPGGPAVANSVARKHGFLNLGQIFGDYYHFWHRGVTKRSLSPHRPRHSRLQREPQVQWLEQQVAKRRTKRDVYQEPTDPKFPQQWYLSGVTQRDLNVKAAWAQGYTGHGIVVSILDDGIEKNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGVCGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLAEEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQIVTTDLRQKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLNANDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKCIIDILTEPKDIGKRLEVRKTVTACLGEPNHITRLEHAQARLTLSYNRRGDLAIHLVSPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVLYGTA

60-75 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH8.0, 20% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.