FZD2 Protein, Human (HEK293, Fc)

FZD2 Protein, a receptor for Wnt proteins, activates the beta-catenin canonical pathway, inhibiting GSK-3 kinase and triggering Wnt target genes. FZD2 and some family members have a secondary PKC-calcium pathway, yet its integration with the canonical pathway remains unclear. PKC seems crucial for Wnt-mediated GSK-3 kinase inactivation. These pathways may involve G-protein interactions, contributing to polarity information transmission during tissue morphogenesis. Notably, FZD2 acts as a receptor for C. difficile toxin TcdB in the colonic epithelium, occupying the Wnt-adducted palmitoleate binding site, preventing downstream Wnt signaling. FZD2 Protein, Human (HEK293, Fc) is the recombinant human-derived FZD2 protein, expressed by HEK293, with C-hFc labeled tag. The total length of FZD2 Protein, Human (HEK293, Fc) is 167 a.a., with molecular weight of 50-62 kDa.

FZD2 Protein functions as a receptor for Wnt proteins, typically engaging the beta-catenin canonical signaling pathway, leading to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin, and subsequent activation of Wnt target genes. Some family members, including FZD2, have demonstrated a secondary signaling pathway involving PKC and calcium fluxes, the integration of which with the canonical pathway remains to be fully elucidated, as PKC appears essential for Wnt-mediated inactivation of GSK-3 kinase. These pathways likely involve interactions with G-proteins and may play roles in transduction and intercellular transmission of polarity information during tissue morphogenesis and in differentiated tissues. Notably, FZD2 acts as a receptor for C.difficile toxin TcdB in the colonic epithelium, where TcdB binding occupies the Wnt-adducted palmitoleate binding site in frizzled receptors, preventing Wnt binding and downstream Wnt signaling.

Human

HEK293

C-hFc

Q14332 (Q24-P190)

2535  [NCBI]

50-62 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.