G-CSFR/CD114 Protein, Human (HEK293)

The G-CSFR/CD114 protein, as a receptor for granulocyte colony-stimulating factor (CSF3), is essential for granulocyte maturation and plays a key role in the proliferation, differentiation, and survival of neutrophil lineage cells. Furthermore, it may be involved in cell surface adhesion or recognition events. G-CSFR/CD114 Protein, Human (HEK293) is the recombinant human-derived G-CSFR/CD114 protein, expressed by HEK293 , with tag free. The total length of G-CSFR/CD114 Protein, Human (HEK293) is 597 a.a., with molecular weight of ~85.36 kDa.

G-CSFR/CD114, the receptor for granulocyte colony-stimulating factor (CSF3), holds a pivotal role in orchestrating granulocytic maturation by governing the proliferation, differentiation, and survival of cells along the neutrophilic lineage. This homodimeric receptor engages with two CSF3 molecules, forming a crucial interaction that regulates essential cellular processes. Beyond its involvement in cellular maturation, G-CSFR/CD114 may contribute to adhesion or recognition events at the cell surface. Furthermore, its interaction with CEACAM1 serves as a regulatory axis, down-regulating the CSF3R-STAT3 pathway by recruiting PTPN6, which subsequently dephosphorylates CSF3R and modulates cellular signaling dynamics.

Measured by its ability to inhibit the G-CSF-induced proliferation of M-NFS‑60 cells. The ED₅₀ for this effect is 1.299 μg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF, corresponding to a specific activity is 769.82 units/mg.

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  Measured by its ability to inhibit the G-CSF-induced proliferation of M-NFS‑60 cells. The ED₅₀ for this effect is 1.299 μg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF, corresponding to a specific activity is 769.82 units/mg.

Human

HEK293

Tag Free

Q99062/NP_000751.1 (E25-P621)

1441  [NCBI]

ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQPGGRQQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAGYPPAIPHNLSCLMNLTTSSLICQWEPGPETHLPTSFTLKSFKSRGNCQTQGDSILDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQLCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQKCELRHKPQRGEASWALVGPLPLEALQYELCGLLPATAYTLQIRCIRWPLPGHWSDWSPSLELRTTERAPTVRLDTWWRQRQLDPRTVQLFWKPVPLEEDSGRIQGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVALVAYNSAGTSRPTPVVFSESRGPALTRLHAMARDPHSLWVGWEPPNPWPQGYVIEWGLGPPSASNSNKTWRMEQNGRATGFLLKENIRPFQLYEIIVTPLYQDTMGPSQHVYAYSQEMAPSHAPELHLKHIGKTWAQLEWVPEPPELGKSPLTHYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHLMAASQAGATNSTVLTLMTLTP

Approximately 85.36 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.