Galectin-1/LGALS1 Protein, Human (His)

Galectin-1/LGALS1 is a lectin that binds β-galactoside and complex carbohydrates and regulates apoptosis, proliferation, and differentiation. It inhibits CD45 phosphatase activity, blocks Lyn kinase dephosphorylation, and induces T cell apoptosis. Galectin-1/LGALS1 Protein, Human (His) is the recombinant human-derived Galectin-1/LGALS1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of Galectin-1/LGALS1 Protein, Human (His) is 134 a.a., with molecular weight of ~15.0 kDa.

The Galectin-1/LGALS1 protein serves as a lectin with the ability to bind beta-galactoside and a diverse range of complex carbohydrates. It plays a pivotal role in the regulation of apoptosis, cell proliferation, and cell differentiation. Galectin-1/LGALS1 exerts its influence by inhibiting the protein phosphatase activity of CD45, consequently impeding the dephosphorylation of Lyn kinase. Additionally, it acts as a potent inducer of T-cell apoptosis. Existing as a homodimer, Galectin-1/LGALS1 forms interactions with a variety of cellular entities, including CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, and CD45. It also binds LGALS3BP, laminin (via poly-N-acetyllactosamine), and SUSD2. Notably, Galectin-1/LGALS1 engages in an interaction with the cargo receptor TMED10, facilitating translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.

CD45 immobilized on CM5 Chip can bind LGALS1 with an affinity constant of 4.859 μM as determined in SPR assay.

Human

E. coli

C-6*His

P09382 (A2-D135)

3956  [NCBI]

ACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD

Approximately 15 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM Citrate, 8% Trehalose, 4% Mannitol, 50 mM NaCl, 1 mM TCEP, 0.05% Tween 80, pH 4.0 or PBS, pH 7.4, 1 mM TCEP, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.