Gamma-hemolysin component A Protein, S. aureus (Myc, His)

Gamma-hemolysin component A (HLgA) functions as a toxin and creates cell membrane pores with hemolytic and leukotoxic activities. Its action depends on sequential binding and cooperative association with class S and class F components to form heterooligomeric complexes. Gamma-hemolysin component A Protein, S. aureus (Myc, His) is the recombinant Staphylococcus aureus-derived Gamma-hemolysin component A protein, expressed by E. coli , with N-His, C-Myc labeled tag. The total length of Gamma-hemolysin component A Protein, S. aureus (Myc, His) is 280 a.a., with molecular weight of ~39.4 kDa.

Gamma-hemolysin component A (HlgA) is a toxin that operates by creating pores in the cell membrane, exhibiting both hemolytic and leucotoxic activities. Its toxic effects are contingent upon the sequential binding and synergistic association of a class S and a class F component, resulting in the formation of heterooligomeric complexes. In strains producing both gamma-hemolysins and leukocidins, HlgA (class S) associates with HlgB (class F), thus constituting an AB toxin. Moreover, HlgA can also form a complex with LukF-PV, showcasing the versatile interactions and potentially cooperative mechanisms employed by this toxin.

Staphylococcus aureus

E. coli

N-His;C-Myc

P0A073 (E30-K309)

59701246

ENKIEDIGQGAEIIKRTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMIWPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNYSKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARDYFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAVDRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK

Approximately 39.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.