GARS Protein, Human (sf9, His)

The GARS protein performs ATP-dependent ligation of glycine to its tRNA, creating a Gly-AMP intermediate. It also produces diadenosine tetraphosphate (Ap4A) by condensing two ATP molecules, which is important for cellular regulation. GARS may have a specific role in maintaining Ap4A homeostasis. GARS Protein, Human (sf9, His) is the recombinant human-derived GARS protein, expressed by Sf9 insect cells , with N-His labeled tag.

GARS, a pivotal enzyme, facilitates the ATP-dependent ligation of glycine to the 3'-end of its corresponding tRNA through the formation of an aminoacyl-adenylate intermediate (Gly-AMP). This process is crucial for protein synthesis and maintaining the fidelity of the genetic code. Additionally, GARS exhibits a unique capability to generate diadenosine tetraphosphate (Ap4A), a universal signaling molecule involved in diverse cell regulation pathways, by directly condensing two ATP molecules. This dual enzymatic activity underscores the multifaceted role of GARS in both fundamental cellular processes, such as protein synthesis, and the modulation of signaling cascades through Ap4A, suggesting its potential significance in Ap4A homeostasis.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

P41250-1/NP_002038.2 (M1-E739)

2617  [NCBI]

Approximately 85.5 kDa

Greater than 85% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, pH 7.5, 150 mM Nacl, 20% Glycerol.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.