1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Ligases (EC 6)
  4. GARS Protein, Human (sf9, His)

GARS Protein, Human (sf9, His)

Cat. No.: HY-P74128
COA Handling Instructions

The GARS protein performs ATP-dependent ligation of glycine to its tRNA, creating a Gly-AMP intermediate. It also produces diadenosine tetraphosphate (Ap4A) by condensing two ATP molecules, which is important for cellular regulation. GARS may have a specific role in maintaining Ap4A homeostasis. GARS Protein, Human (sf9, His) is the recombinant human-derived GARS protein, expressed by Sf9 insect cells , with N-His labeled tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
10 μg $115 In-stock
50 μg $320 In-stock
100 μg $510 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

The GARS protein performs ATP-dependent ligation of glycine to its tRNA, creating a Gly-AMP intermediate. It also produces diadenosine tetraphosphate (Ap4A) by condensing two ATP molecules, which is important for cellular regulation. GARS may have a specific role in maintaining Ap4A homeostasis. GARS Protein, Human (sf9, His) is the recombinant human-derived GARS protein, expressed by Sf9 insect cells , with N-His labeled tag.

Background

GARS, a pivotal enzyme, facilitates the ATP-dependent ligation of glycine to the 3'-end of its corresponding tRNA through the formation of an aminoacyl-adenylate intermediate (Gly-AMP). This process is crucial for protein synthesis and maintaining the fidelity of the genetic code. Additionally, GARS exhibits a unique capability to generate diadenosine tetraphosphate (Ap4A), a universal signaling molecule involved in diverse cell regulation pathways, by directly condensing two ATP molecules. This dual enzymatic activity underscores the multifaceted role of GARS in both fundamental cellular processes, such as protein synthesis, and the modulation of signaling cascades through Ap4A, suggesting its potential significance in Ap4A homeostasis.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

Sf9 insect cells

Tag

N-His

Accession

P41250-1/NP_002038.2 (M1-E739)

Gene ID
Molecular Construction
N-term
His
GARS (M1-E739)
Accession # P41250-1/NP_002038.2
C-term
Synonyms
Glycine--tRNA ligase; GlyRS; GARS1; GARS
Molecular Weight

Approximately 85.5 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, pH 7.5, 150 mM Nacl, 20% Glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

GARS Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GARS Protein, Human (sf9, His)
Cat. No.:
HY-P74128
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