GAS6 Protein, Mouse (HEK293, His, solution)

GAS6 Protein is a vitamin K-dependent protein involved in signaling pathways including GAS6/AXL. The GAS6 Protein contains an NH₂ terminal Gla domain as well as four EGF-like repeats and tandem gl bular domains. GAS6 Protein, Mouse (HEK293, His, solution) is the recombinant mouse-derived GAS6 protein, expressed by HEK293 , with C-His labeled tag.

Growth arrest specific protein 6 (GAS6) is a vitamin K-dependent protein that has a high degree of structural homology with natural anticoagulant S, having the same modular composition and 40% sequence homology. GAS6 contains an NH₂ terminal Gla domain as well as four EGF-like repeat sequences and tandem globular domains. GAS6 is a ligand for the tyrosine-protein kinase receptors AXL, TYRO3, and MER, whose signaling is involved in cell growth and survival, cell adhesion, and cell migration. GAS6/AXL signaling plays a role in a variety of processes, such as the survival of endothelial cells during acidification, by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, liver regeneration, survival and migration of gonadotropin-releasing hormone neurons, platelet activation or regulation of thrombotic response^{[1][2][3][4][5]}.

Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. The ED₅₀ for this effect is 45.93 ng/mL. Corresponding to a specific activity is 21772.262 U/mg.

Mouse

HEK293

C-His

Q61592 (V28-P674)

14456  [NCBI]

VLLRAREAAQFLRPRQRRAYQVFEEAKQGHLERECVEEVCSKEEAREVFENDPETEYFYPRYQECMRKYGRPEEKNPDFAKCVQNLPDQCTPNPCDKKGTHICQDLMGNFFCVCTDGWGGRLCDKDVNECVQKNGGCSQVCHNKPGSFQCACHSGFSLASDGQTCQDIDECTDSDTCGDARCKNLPGSYSCLCDEGYTYSSKEKTCQDVDECQQDRCEQTCVNSPGSYTCHCDGRGGLKLSPDMDTCEDILPCVPFSMAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLLAEFDFRTFDPEGVLFFAGGRSDSTWIVLGLRAGRLELQLRYNGVGRITSSGPTINHGMWQTISVEELERNLVIKVNKDAVMKIAVAGELFQLERGLYHLNLTVGGIPFKESELVQPINPRLDGCMRSWNWLNGEDSAIQETVKANTKMQCFSVTERGSFFPGNGFATYRLNYTRTSLDVGTETTWEVKVVARIRPATDTGVLLALVGDDDVVPISVALVDYHSTKKLKKQLVVLAVEDVALALMEIKVCDSQEHTVTVSLREGEATLEVDGTKGQSEVSTAQLQERLDTLKTHLQGSVHTYVGGLPEVSVISAPVTAFYRGCMTLEVNGKILDLDTASYKHSDITSHSCPPVEHATP

Approximately 73.5-95 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of sterile PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.