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GAS6 Protein, Human (HEK293, Fc)

Cat. No.: HY-P70470
COA Handling Instructions

GAS6 Protein is a secreted protein located outside the cell membrane. GAS6 Protein is a ligand for tyrosine protein kinase receptors AXL, TYRO3, and MER, and its signal transduction is related to cell growth and survival, cell adhesion, and cell migration. GAS6 is associated with tumor cell growth, metastasis, invasion, epithelial mesenchymal transition, angiogenesis, drug resistance, immune regulation, and stem cell maintenance after binding to AXL. GAS6 Protein, Human (HEK293, Fc) is a recombinant GAS6 protein with an Fc tag, expressed by HEK293.

For research use only. We do not sell to patients.

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10 μg $135 In-stock
50 μg $380 In-stock
100 μg $645 In-stock
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  • Biological Activity

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Description

GAS6 Protein is a secreted protein located outside the cell membrane. GAS6 Protein is a ligand for tyrosine protein kinase receptors AXL, TYRO3, and MER, and its signal transduction is related to cell growth and survival, cell adhesion, and cell migration. GAS6 is associated with tumor cell growth, metastasis, invasion, epithelial mesenchymal transition, angiogenesis, drug resistance, immune regulation, and stem cell maintenance after binding to AXL. GAS6 Protein, Human (HEK293, Fc) is a recombinant GAS6 protein with an Fc tag, expressed by HEK293[1][2].

Background

GAS6 Protein is encoded by the growth arrest-specific gene 6 (Gas6) and belongs to the vitamin K-dependent protein family[2].
GAS6 Protein-AXL can regulate various signaling pathways like PI3K/AKT/mTOR, JAK/STAT, NF-κB, and RAS/RAF/MEK/ERK. It plays a role in tumor cell survival, anti-apoptotic signaling, mitosis, migration, invasion, drug resistance, angiogenesis, and the tumor-host relationship[2].
GAS6 Protein-AXL can promote the survival of endothelial cells, regulate the optimal cytokine signaling during the development of human natural killer cells, support liver regeneration, assist the survival and migration of gonadotropin-releasing hormone neurons, and facilitate platelet activation or thrombotic responses; it can also induce actin remodeling, driving membrane folding, giant cell phagocytosis, and cancer cell invasion[1][3].
GAS6 Protein signaling plays a beneficial role in septic heart conditions by inhibiting inflammation, oxidative stress, mitochondrial damage, and ER stress, with NLRP3 being the main molecule mediating GAS6 signaling[4].
GAS6 Protein mediates the binding of viruses to target cells by bridging the phosphatidylserine on the viral particle to the TAM receptor tyrosine kinase AxI on the target cell, facilitating viral entry[5].
GAS6 Protein is closely related to pathological processes like nephritis, tumors, viral infections, and cardiovascular diseases[1][4][5].

In Vitro

GAS6 Protein can trigger membrane folding in human glioblastoma LN229 cells, manifested as the formation of peripheral folds (PR) and circular dorsal folds (CDR)[1].
GAS6 Protein (25-100 ng/mL, 3 h) can inhibit the expression of inflammatory factors and NLRP3, ASC proteins, thereby reducing LPS induced inflammation in HL-1 cells[4].

In Vivo

Loss of GAS6 Protein expression in GAS6 knockout (GAS6 KO) mice leads to exacerbation of septic myocardial injury in septic mice[4].
GAS6 Protein (2 μg; once daily; 10 days; i.p.) induces erythropoiesis and alleviates anemia in GAS6-/-anemic mice[6].
GAS6 Protein (2 μg; once daily; 4 days; injection) can increase proteinuria and the number of PCNA positive cells in each glomerulus of GAS6-/-nephrotoxic nephritis mice, thereby promoting the development of nephritis[7].

Biological Activity

Immobilized Human AXL-His at 5 μg/mL (100 μl/well) can bind Human GAS6-Fc. The ED50 of Human GAS6-Fc is ≤27 μg/mL.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

Q14393-2 (A31-A678)

Gene ID
Molecular Construction
N-term
GAS6 (A31-A678)
Accession # Q14393-2
hFc
C-term
Synonyms
AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6
AA Sequence

ALLPAREATQFLRPRQRRAFQVFEEAKQGHLERECVEELCSREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNFFCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEARCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDILPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA

Molecular Weight

110-120 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

GAS6 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GAS6 Protein, Human (HEK293, Fc)
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