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  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. GCDH Protein, Human (sf9, His)

GCDH Protein, Human (sf9, His)

Cat. No.: HY-P76357
Handling Instructions

The GCDH protein is essential for the degradation of L-lysine, L-hydroxylysine, and L-tryptophan. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA, releasing CO(2). GCDH Protein, Human (sf9, His) is the recombinant human-derived GCDH protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The GCDH protein is essential for the degradation of L-lysine, L-hydroxylysine, and L-tryptophan. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA, releasing CO(2). GCDH Protein, Human (sf9, His) is the recombinant human-derived GCDH protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

GCDH Protein plays a crucial role in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism by catalyzing the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA, accompanied by the release of CO(2). This enzymatic process involves the utilization of electron transfer flavoprotein as its electron acceptor, highlighting the protein's dependence on this cofactor for efficient functioning within the metabolic pathway. It's noteworthy that the Short isoform of GCDH Protein is inactive, indicating the existence of multiple isoforms with varying functional properties in the regulation of this metabolic process.

Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

Q92947-1/NP_000150.1 (R45-K438)

Gene ID
Molecular Construction
N-term
GCDH (R45-K438)
Accession # Q92947-1/NP_000150.1
His
C-term
Synonyms
Glutaryl-CoA Dehydrogenase Mitochondrial; GCD; GCDH
Molecular Weight

Approximately 44.9 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

GCDH Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GCDH Protein, Human (sf9, His)
Cat. No.:
HY-P76357
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