1. Recombinant Proteins
  2. Fluorescent-labeled Recombinant Proteins
  3. GFP Protein, Aequorea victoria (His)

GFP Protein, Aequorea victoria (His)

Cat. No.: HY-P71461
COA Handling Instructions

The green fluorescent protein (GFP), Aequorea victoria is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range, found in organisms including Aequorea victoria, corals, sea anemones, zoanithids, copepods and lancelets. GFP Protein has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm, while its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. GFP Protein acts as an energy transfer receptor and actively transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer, which is used as expression of reporter genes. GFP Protein, Aequorea victoria (His) is a recombinant jellyfish Aequorea victoria GFP protein with N-6*His labeled tag that consists of 238 amino acids, which is expressed in E. coli .

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
5 μg $30 In-stock
10 μg $48 In-stock
50 μg $125 In-stock
100 μg $200 In-stock
500 μg $600 Get quote
> 500 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

The green fluorescent protein (GFP), Aequorea victoria is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range, found in organisms including Aequorea victoria, corals, sea anemones, zoanithids, copepods and lancelets. GFP Protein has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm, while its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. GFP Protein acts as an energy transfer receptor and actively transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer, which is used as expression of reporter genes. GFP Protein, Aequorea victoria (His) is a recombinant jellyfish Aequorea victoria GFP protein with N-6*His labeled tag that consists of 238 amino acids, which is expressed in E. coli [1][2][3][4][5][6][7].

Background

GFP fluorescence occurs when aequorin interacts with Ca2+ ions, inducing a blue glow in Aequorea victoria. Some of this luminescent energy is transferred to the GFP, shifting the overall color towards green, making GFP protein enabling the visualization of specific proteins and cellular structures in living organisms[3].
GFP Protein has a β barrel structure consisting of eleven β-strands with a pleated sheet arrangement, with an α helix containing the covalently bonded chromophore 4-(p-hydroxybenzylidene)imidazolidin-5-one (HBI) running through the center[4].
In a live cell imaging experiment, on the one hand, GFP Protein directly attaches to a protein of interest to indicate a successful transfection of a gene of interest. On the other hand, GFP Protein that containing a mutation where the fluorescence will change from green to yellow over time is used to study the state of protein production such as recently activated, continuously activated or recently deactivated[6].
GFP Protein is used widely in cancer research to label and track cancer cells. GFP Protein-labelled cancer cells have been used to model metastasis, the process by which cancer cells spread to distant organs[7].

In Vitro

GFP Protein (Aequorea victoria) acts as a pollutant marker. There is a decrease in both GFP Protein and cellular density as pollutant levels increases, indicating that cellular activity has decreased[5].

In Vivo

Zebrafish that are injected with GFP Protein (Aequorea victoria) are approximately twenty times more susceptible to recognize cellular stresses than zebrafish that are not injected with GFP Protein (Aequorea victoria)[5].
Tumor dissemination and metastasis occur at distinct times during tumor progression in the bi-transgenic MMTV-PyMT and β-actin-enhanced green fluorescent protein (GFP) mice[7].

Species

Others

Source

E. coli

Tag

N-6*His

Accession

P42212 (M1-K238)

Gene ID

/

Molecular Construction
N-term
6*His
GFP (M1-K238)
Accession # P42212
C-term
Synonyms
GFPGreen fluorescent protein
AA Sequence

MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK

Molecular Weight

27-31 kDa

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 or 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween 80, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 1 year, protect from light. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

GFP Protein, Aequorea victoria (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
GFP Protein, Aequorea victoria (His)
Cat. No.:
HY-P71461
Quantity:
MCE Japan Authorized Agent: