GFRA1/GDNFR-alpha-1 Protein, Human (HEK293, His)

The GFRA1/GDNFR-alpha-1 Protein serves as a receptor for GDNF, facilitating GDNF-induced autophosphorylation and activation of the RET receptor. In a complex, two molecules of GDNFR-alpha-1 are believed to associate with the disulfide-linked GDNF dimer and two molecules of RET. It interacts with RET and SORL1, either alone or in a complex with GDNF, leading to GFRA1 internalization without degradation. GFRA1/GDNFR-alpha-1 Protein, Human (HEK293, His) is the recombinant human-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-6*His labeled tag.

GFRA1/GDNFR-alpha-1, a receptor for Glial Cell Line-Derived Neurotrophic Factor (GDNF), plays a crucial role in mediating GDNF-induced autophosphorylation and activation of the RET receptor. The proposed model suggests that two molecules of GDNFR-alpha-1 form a complex with the disulfide-linked GDNF dimer and two molecules of RET. Interaction studies confirm direct binding with RET, facilitating GDNF signaling. Furthermore, GFRA1 engages with SORL1, either independently or in complex with GDNF, leading to the internalization of GFRA1 without degradation. This intricate interplay highlights the regulatory mechanisms involved in GDNF-mediated cellular responses, shedding light on the multifaceted roles of GFRA1 in coordinating signaling events critical for cellular homeostasis and development.

Human

HEK293

C-6*His

P56159-2 (D25-K429)

2674  [NCBI]

DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLASGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGLGASSHITTK

Approximately 60.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.