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  3. GIPR Protein, Mouse (HEK293, His)

GIPR Protein, Mouse (HEK293, His)

Cat. No.: HY-P702801
COA Handling Instructions

AK2 protein plays a key role in cellular energy homeostasis and adenine nucleotide metabolism by catalyzing the reversible transfer of terminal phosphate groups between ATP and AMP. Its adenylate kinase activity plays a key role in regulating phosphate utilization and de novo AMP biosynthetic pathways, contributing to the complex balance of cellular energy resources. GIPR Protein, Mouse (HEK293, His) is the recombinant mouse-derived GIPR, expressed by HEK293 , with C-10*His labeled tag. The total length of GIPR Protein, Mouse (HEK293, His) is 116 a.a.,

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $230 In-stock
50 μg $435 In-stock
100 μg $700 Get quote
> 100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

AK2 protein plays a key role in cellular energy homeostasis and adenine nucleotide metabolism by catalyzing the reversible transfer of terminal phosphate groups between ATP and AMP. Its adenylate kinase activity plays a key role in regulating phosphate utilization and de novo AMP biosynthetic pathways, contributing to the complex balance of cellular energy resources. GIPR Protein, Mouse (HEK293, His) is the recombinant mouse-derived GIPR, expressed by HEK293 , with C-10*His labeled tag. The total length of GIPR Protein, Mouse (HEK293, His) is 116 a.a.,

Background

Adenylate Kinase 2 (AK2) is an enzyme that catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP, a pivotal reaction in cellular energy homeostasis and adenine nucleotide metabolism. The adenylate kinase activity of AK2 is crucial for regulating phosphate utilization and the de novo biosynthesis of AMP, key processes in cellular energy management. Beyond its role in energy metabolism, AK2 also plays a significant role in hematopoiesis, emphasizing its importance in blood cell development. The multifaceted functions of AK2 underscore its critical role in maintaining cellular energy balance, nucleotide synthesis, and supporting fundamental biological processes such as hematopoiesis.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized GIPR, Mouse (HEK293, His) at 2 μg/mL can bind Anti-Mouse Gipr Recombinant Antibody, the EC50 is ≤30 ng/mL.

Species

Mouse

Source

HEK293

Tag

C-10*His

Accession

P54819-1 (E19-Q134)

Gene ID

381853

Synonyms
Gastric inhibitory polypeptide receptor; Glucose-dependent insulinotropic polypeptide receptor
Molecular Weight

32 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. or PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

GIPR Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GIPR Protein, Mouse (HEK293, His)
Cat. No.:
HY-P702801
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