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Glial fibrillary acidic Protein, Human (432a.a, His)

Cat. No.: HY-P702496
COA Handling Instructions

Glial fibrillary acidic protein (GFAP) belongs to the class III intermediate filament family and serves as a cell-specific marker that is critical for distinguishing astrocytes from other glial cells during the development of the central nervous system. GFAP plays a key role in differentiating the identity of astrocytes, contributing to their unique characteristics. Glial fibrillary acidic Protein, Human (432a.a, His) is the recombinant human-derived Glial fibrillary acidic protein, expressed by E. coli , with N-6*His labeled tag. The total length of Glial fibrillary acidic Protein, Human (432a.a, His) is 432 a.a., with molecular weight of 54.0 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $180 In-stock
50 μg $350 In-stock
100 μg $600 Get quote
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Description

Glial fibrillary acidic protein (GFAP) belongs to the class III intermediate filament family and serves as a cell-specific marker that is critical for distinguishing astrocytes from other glial cells during the development of the central nervous system. GFAP plays a key role in differentiating the identity of astrocytes, contributing to their unique characteristics. Glial fibrillary acidic Protein, Human (432a.a, His) is the recombinant human-derived Glial fibrillary acidic protein, expressed by E. coli , with N-6*His labeled tag. The total length of Glial fibrillary acidic Protein, Human (432a.a, His) is 432 a.a., with molecular weight of 54.0 kDa.

Background

Glial Fibrillary Acidic Protein (GFAP), classified as a class-III intermediate filament, serves as a cell-specific marker crucial for distinguishing astrocytes from other glial cells, particularly during the central nervous system's developmental stages. As a key structural component of astrocytes, GFAP contributes to the formation and maintenance of the cytoskeleton, providing structural support and stability. Additionally, GFAP is recognized as a reliable marker for identifying and characterizing astroglial cells in various physiological and pathological contexts. It has been reported to interact with SYNM, indicating potential functional connections between GFAP and other proteins involved in cytoskeletal dynamics or cellular signaling within the astrocyte. Ongoing research may provide further insights into the multifaceted roles of GFAP in astrocyte biology and central nervous system development.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

P14136 (M1-M432)

Gene ID

2670

Molecular Construction
N-term
6*His
GFAP (M1-M432)
Accession # P14136
C-term
Synonyms
rHuGlial fibrillary acidic protein/GFAP, His; Glial Fibrillary Acidic Protein; GFAP
Molecular Weight

54.0 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Glial fibrillary acidic Protein, Human (432a.a, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Glial fibrillary acidic Protein, Human (432a.a, His)
Cat. No.:
HY-P702496
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